Gastroenterology Department, The Third Affiliated Hospital of Guangzhou Medical University, Guang Zhou, 510150, China,
Med Oncol. 2013 Dec;30(4):704. doi: 10.1007/s12032-013-0704-7. Epub 2013 Aug 29.
Colorectal cancer is the third most common malignancy worldwide. 5-fluorouracil (5-FU) is the commonly used chemotherapeutic agent, however, more patients develop resistance. Phosphatidylinositol 3-kinases (PI3Ks) play a crucial role in a wide range of cellular processes associated with malignant behavior including cell growth, migration, and survival. In this study, we show increased expression of PI3K p85α during the progression of colorectal cancer. Silencing of PI3K p85α in colorectal cancer cells increased disruption of mitochondrial membrane potential and enhanced 5-FU-induced apoptosis. Furthermore, PI3K p85α-depletion results in activated expression of apoptosis-associated genes Bcl-6, Bim, and Bax. Our results suggest that knockdown of PI3K p85α is a potential therapeutic strategy in the treatment of colorectal cancer.
结直肠癌是全球第三大常见恶性肿瘤。氟尿嘧啶（5-FU）是常用的化疗药物，但更多患者会产生耐药性。磷脂酰肌醇 3-激酶（PI3Ks）在与恶性行为相关的多种细胞过程中发挥关键作用，包括细胞生长、迁移和存活。在这项研究中，我们发现结直肠癌进展过程中 PI3K p85α 的表达增加。在结直肠癌细胞中沉默 PI3K p85α 会增加线粒体膜电位的破坏，并增强 5-FU 诱导的细胞凋亡。此外，PI3K p85α 的耗竭会导致凋亡相关基因 Bcl-6、Bim 和 Bax 的激活表达。我们的研究结果表明，敲低 PI3K p85α 可能是治疗结直肠癌的一种潜在治疗策略。
