Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Food Chem. 2013 Dec 15;141(4):3703-13. doi: 10.1016/j.foodchem.2013.06.003. Epub 2013 Jun 10.
Piper betel leaf (PBL) has the biological capabilities of detoxification and can work as an anti-inflammatory agent and an anti-oxidant. In this study, we evaluated the anti-oxidative activity of the extract of Piper betel leaves (PBLs) on the basis of Cu(2+)-mediated oxidation, and its ability to prevent foam cell formation in a model for oxidised low density lipoprotein (oxLDL)-induced lipid accumulation in macrophages. Our data demonstrated that PBLs were able to inhibit LDL oxidation in vitro and are able to reduce the lipid accumulation in macrophages. We showed the underlying mechanisms to be the following: PBLs up-regulated the protein levels of the class A and class B scavenger receptors, the membrane lipid transporter ABCA1, and its upstream regulator Liver X receptor (LXR) in the macrophages exposed to oxLDL. The results suggested that PBLs activated the reverse cholesterol transport mechanism to enhance the metabolism of the oxLDL that could prevent both lipid accumulation and foam cell formation and further minimise the possible damage of vessels caused by the oxLDL.
荜茇叶（Piper betel leaf，PBL）具有解毒作用，可作为抗炎剂和抗氧化剂。在这项研究中，我们基于 Cu(2+)介导的氧化，评估了荜茇叶提取物（PBLs）的抗氧化活性，并评估了其在氧化型低密度脂蛋白（oxLDL）诱导的巨噬细胞脂质积累模型中预防泡沫细胞形成的能力。我们的数据表明，PBLs 能够抑制 LDL 的体外氧化，并能够减少巨噬细胞中的脂质积累。我们表明，其潜在机制如下：PBLs 上调了暴露于 oxLDL 的巨噬细胞中 A 类和 B 类清道夫受体、膜脂质转运蛋白 ABCA1 及其上游调节因子肝 X 受体（LXR）的蛋白水平。结果表明，PBLs 激活了胆固醇逆转运机制，增强了 oxLDL 的代谢，从而可以预防脂质积累和泡沫细胞形成，并进一步减少 oxLDL 可能对血管造成的损害。
