Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, PR China.
Food Chem. 2013 Dec 15;141(4):3947-53. doi: 10.1016/j.foodchem.2013.06.024. Epub 2013 Jun 15.
A novel sensor for the rapid and label-free detection of imidacloprid was developed based on the combination of a colloidal crystal templating method and a molecular imprinting technique. The molecular imprinted photonic hydrogel film was prepared with methacrylic acid as monomers, ethylene glycol dimethylacrylate as cross-linkers and imidacloprid as imprinting template molecules. When the colloidal crystal template and the molecularly imprinted template was removed, the resulted MIPH film possessed a highly ordered three-dimensional macroporous structure with nanocavities. The response of the MIPH film to imidacloprid in aqueous solution can be detected through a readable Bragg diffraction red shift. When the concentration of imidacloprid increased from 10(-13) to 10(-7) g/mL, the Bragg diffraction peak shifted from 551 to 589 nm, while there were no obvious peak shifts for thiamethoxam and acetamiprid. This sensor which comprises of no label techniques and expensive instruments has potential application for the detection of trace imidacloprid.
基于胶体晶体模板法和分子印迹技术，开发了一种用于快速、无标记检测吡虫啉的新型传感器。以甲基丙烯酸为单体、乙二醇二甲基丙烯酸酯为交联剂、吡虫啉为印迹模板分子，制备了分子印迹光子水凝胶膜。当去除胶体晶体模板和分子印迹模板时，所得 MIPH 膜具有高度有序的三维大孔结构和纳米腔。MIPH 膜对水溶液中吡虫啉的响应可以通过可读布拉格衍射红移来检测。当吡虫啉的浓度从 10(-13)增加到 10(-7)g/mL 时，布拉格衍射峰从 551nm 移动到 589nm，而噻虫嗪和乙酰甲胺磷没有明显的峰移动。该传感器不包含标记技术和昂贵的仪器，具有检测痕量吡虫啉的潜在应用。
