Departament de Fisiologia and Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Facultat de Farmàcia, Universitat de Barcelona (UB), Av. Joan XXIII s/n, 08028 Barcelona, Spain.
Food Chem. 2013 Dec 15;141(4):4375-81. doi: 10.1016/j.foodchem.2013.07.023. Epub 2013 Jul 12.
Maslinic acid, a pentacyclic triterpene from Olea europaea L., exerts hypoglycemic, antioxidant, cardioprotective and antitumoral activities. Here, an LC-APCI-MS method to determine this compound in plasma is presented as a first step in pharmacokinetic studies. Maslinic acid was extracted from plasma with ethyl acetate and separated on a C18 column using a gradient elution of water and acetonitrile. The target ions were m/z 471.3 for maslinic acid and m/z 455.3 for I.S. The method was validated by the analysis of spiked plasma samples obtaining a linear correlation, a recovery of 99.0±0.9% and a quantification limit of 5 nM. The precision and accuracy were ≤8.38% and ≤4.82%, respectively. Finally, the method was verified by measuring the rat plasmatic concentrations 24h after the single oral administration of 10, 25 and 50 mg/kg of maslinic acid, thereby ensuring that the procedure is appropriate for its use in bioavailability studies.
从欧洲油橄榄中提取的五环三萜酸 maslinic acid 具有降血糖、抗氧化、心脏保护和抗肿瘤活性。本文介绍了一种用于测定血浆中这种化合物的 LC-APCI-MS 方法，这是药代动力学研究的第一步。maslinic acid 用乙酸乙酯从血浆中提取，然后在 C18 柱上用梯度洗脱水和乙腈进行分离。目标离子为 m/z 471.3（maslinic acid）和 m/z 455.3（IS）。该方法通过分析加标血浆样品进行验证，得到线性相关、回收率为 99.0±0.9%和定量限为 5 nM。精密度和准确度分别为≤8.38%和≤4.82%。最后，通过测量大鼠单次口服 10、25 和 50 mg/kg maslinic acid 24 小时后的血浆浓度验证了该方法，从而确保该程序适用于生物利用度研究。
