Institut für Molekulare Biowissenschaften, Johann Wolfgang Goethe-Universität Frankfurt, Max-von-Laue-Str. 9, D-60438 Frankfurt/M., Germany.
Bioorg Med Chem. 2013 Oct 15;21(20):6233-8. doi: 10.1016/j.bmc.2013.07.035. Epub 2013 Jul 31.
RNA aptamers have been widely used as regulators for conditional gene expression. The TetR binding aptamer can activate tetracycline repressor TetR controlled gene expression with high efficiency. Here we demonstrate that the aptamer can also activate TetR controlled gene expression when expressed in the context of a natural transcripts. The aptamer was inserted into the untranslated regions of mRNAs as well as into small non-coding RNAs and was expressed both from a plasmid and from an endogenous locus. Our data suggest that the aptamer is a valuable tool to easily monitor the expression level of different RNAs, and it therefore represents a powerful tool for the construction of complex synthetic gene networks.
RNA 适体已被广泛用作条件基因表达的调节剂。TetR 结合适体能高效激活四环素阻遏物 TetR 控制的基因表达。在这里，我们证明了该适体在天然转录本的背景下也可以激活 TetR 控制的基因表达。该适体被插入到 mRNA 的非翻译区以及小非编码 RNA 中，并从质粒和内源性基因座表达。我们的数据表明，该适体是一种监测不同 RNA 表达水平的有用工具，因此它代表了构建复杂合成基因网络的有力工具。
