Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada.
Prostaglandins Leukot Essent Fatty Acids. 2013 Sep;89(4):169-77. doi: 10.1016/j.plefa.2013.08.002. Epub 2013 Aug 13.
The goal of the present study was to determine whether endogenous synthesis of n-3 polyunsaturated fatty acids (PUFA) in the fat-1 mouse is comparable to fish oil feeding with respect to kidney n-3 PUFA composition and eicosanoid levels. Wild-type and heterozygous fat-1 mice, capable of synthesizing n-3 PUFA endogenously, were given diets enriched in either n-3 or n-6 PUFA in a 2×2 factorial design and terminated after 12 weeks. Kidney phospholipid fatty acids were analysed by gas chromatography. Kidney eicosanoids were analysed by liquid chromatography tandem mass spectrometry. Relative to control mice fed n-6 PUFA, n-3 PUFA fed and fat-1 mice had higher levels of kidney phospholipid n-3 PUFA, and lower levels of n-6 PUFA and eicosanoids. However, mice fed n-3 PUFA mice had higher levels of n-3 PUFA and lower levels of eicosanoids as compared to fat-1 mice. In conclusion, diet feeding had a greater impact on kidney fatty acid composition and eicosanoid levels than the genetic effect of the fat-1 gene. However, the fat-1 mouse remains a close approximation that can be used as a complementary model to study the role of n-3 PUFA in the kidney.
本研究的目的是确定与鱼油喂养相比，fat-1 小鼠体内内源性合成 n-3 多不饱和脂肪酸 (PUFA) 是否在肾脏 n-3 PUFA 组成和类二十烷酸水平方面具有可比性。能够内源性合成 n-3 PUFA 的野生型和杂合 fat-1 小鼠以 2×2 析因设计接受富含 n-3 或 n-6 PUFA 的饮食，并在 12 周后终止。通过气相色谱法分析肾脏磷脂脂肪酸。通过液相色谱串联质谱法分析肾脏类二十烷酸。与摄入 n-6 PUFA 的对照小鼠相比，摄入 n-3 PUFA 和 fat-1 小鼠的肾脏磷脂 n-3 PUFA 水平更高，n-6 PUFA 和类二十烷酸水平更低。然而，与 fat-1 小鼠相比，摄入 n-3 PUFA 的小鼠的 n-3 PUFA 水平更高，类二十烷酸水平更低。总之，饮食对肾脏脂肪酸组成和类二十烷酸水平的影响大于 fat-1 基因的遗传效应。然而，fat-1 小鼠仍然是一种接近的近似值，可以用作补充模型来研究 n-3 PUFA 在肾脏中的作用。
