Department of Laboratory Medicine, School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata 700 073, India.
Cell Immunol. 2013 Jul-Aug;284(1-2):172-81. doi: 10.1016/j.cellimm.2013.07.020. Epub 2013 Aug 8.
T11 target structure (T11TS), a membrane glycoprotein has been documented with anti neoplastic activity in glioma bearing animal model in our lab. In this study, we have evaluated the phagocytic potential, expression of VEGF, TNF-α in T11TS treated and untreated macrophages in all four grades of glioma. The data indicates the significant enhancement of phagocytosis in T11TS treated macrophages of grades I and II glioma. There was significant up regulation in TNF-α and significant down regulation in VEGF expression in T11TS treated macrophages in grade I and II glioma. We also attempted to know any possible apoptotic role of T11TS in tumor cells by comparing Bax and Bcl2 in treated and untreated tumor cells of all four grades. We found significant up regulation in Bax expression and down regulation in Bcl2 expression of grades I and II glioma. The outcome may help in pushing this molecule into pharmaceutical domain.
我们实验室的动物模型研究表明，T11 靶结构（T11TS）是一种膜糖蛋白，具有抗肿瘤活性。在这项研究中，我们评估了吞噬作用、血管内皮生长因子（VEGF）和肿瘤坏死因子-α（TNF-α）在 T11TS 处理和未处理的巨噬细胞中的表达，这四种巨噬细胞均来自于四个级别的神经胶质瘤。数据表明，T11TS 处理的 I 级和 II 级神经胶质瘤的巨噬细胞的吞噬作用明显增强。在 I 级和 II 级神经胶质瘤中，T11TS 处理的巨噬细胞中 TNF-α 的表达显著上调，VEGF 的表达显著下调。我们还试图通过比较四个级别中治疗和未治疗的肿瘤细胞中的 Bax 和 Bcl2，了解 T11TS 在肿瘤细胞中是否存在任何可能的凋亡作用。我们发现 I 级和 II 级神经胶质瘤中 Bax 的表达显著上调，Bcl2 的表达显著下调。研究结果可能有助于推动这一分子进入制药领域。
