Konttinen Y T, Kulomaa M, Segerberg-Konttinen M, Nordström D, Keinänen R, Grönblad M, Malmström M
Fourth Department of Medicine, Helsinki University Central Hospital, Finland.
Scand J Dent Res. 1990 Aug;98(4):318-25. doi: 10.1111/j.1600-0722.1990.tb00979.x.
In this study, lysozyme mRNA in labial salivary glands has been localized with in situ hybridization technique using 35S-labeled hen lysozyme cDNA (cDNALZM) as a hybridization probe in normals and in patients with Sjögren's syndrome, 35S-DNALZM:mRNA hybrids were detected only in acinar serous cells, although lysozyme was identified in ductal cells using immunohistochemical techniques. Our results suggest that the serous acinar cells are the only site of lysozyme synthesis in small salivary glands. The presence of lysozyme in ductal cells may be a result of reabsorption from the saliva or concentration from the blood or surrounding tissues.
在本研究中，采用原位杂交技术，以35S标记的鸡溶菌酶cDNA（cDNALZM）作为杂交探针，对正常人和干燥综合征患者唇腺中的溶菌酶mRNA进行了定位。尽管用免疫组化技术在导管细胞中鉴定出了溶菌酶，但仅在腺泡浆液细胞中检测到35S-DNALZM:mRNA杂交体。我们的结果表明，浆液性腺泡细胞是小唾液腺中溶菌酶合成的唯一部位。导管细胞中溶菌酶的存在可能是唾液重吸收、血液或周围组织浓缩的结果。
