Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
Fish Shellfish Immunol. 2013 Nov;35(5):1670-6. doi: 10.1016/j.fsi.2013.08.010. Epub 2013 Aug 28.
Thioredoxin (abbreviated as Trx) is an important ubiquitous disulfide reductase, which can protect organisms against various oxidative stresses. In the present study, a thioredoxin-related protein of 14 kDa (named as Ec-TRP14) was identified from the marine fish grouper, Epinephelus coioides by RACE PCR. The full-length cDNA of Ec-TRP14 was comprised of 1066 bp with a 372 bp open reading frame that encodes a putative protein of 123 amino acids. Similar to most TRP14s, Ec-TRP14 contained the conserved motif C-P-D-C. Ec-TRP14 mRNA is predominately expressed in liver, brain and muscle. The expression of Ec-TRP14 was up-regulated in the liver of grouper challenged with SGIV. Ec-TRP14 was recombined and expressed in Escherichia coli BL21 (DE3), and the rEc-Ec-TRP14 fusion protein was demonstrated to possess the antioxidant activity. The grouper spleen (GS) cells were treated with a high concentration of rEc-TRP14 (8.3 μg/ml), which significantly enhanced cells viability under damage caused by viral infection. These results together indicated that Ec-TRP14 could function as an important antioxidant in a physiological context, and might be involved in the responses to viral challenge.
硫氧还蛋白（Trx）是一种重要的普遍存在的二硫键还原酶，可以保护生物免受各种氧化应激。本研究通过 RACE PCR 从海水鱼石斑鱼（Epinephelus coioides）中鉴定出一种 14 kDa 的硫氧还蛋白相关蛋白（命名为 Ec-TRP14）。Ec-TRP14 的全长 cDNA 由 1066 bp 组成，其中 372 bp 开放阅读框编码一个 123 个氨基酸的假定蛋白。与大多数 TRP14 一样，Ec-TRP14 含有保守的 C-P-D-C 基序。Ec-TRP14 mRNA 主要在肝脏、大脑和肌肉中表达。在石斑鱼受到 SGIV 攻击时，Ec-TRP14 在肝脏中的表达上调。Ec-TRP14 在大肠杆菌 BL21（DE3）中重组表达，rEc-Ec-TRP14 融合蛋白表现出抗氧化活性。用高浓度 rEc-TRP14（8.3 μg/ml）处理石斑鱼脾脏（GS）细胞，可显著提高病毒感染引起的细胞活力。这些结果表明，Ec-TRP14 在生理条件下可作为一种重要的抗氧化剂，并可能参与对病毒攻击的反应。
