Department of Mechanical Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
J Biotechnol. 2013 Oct 20;168(2):135-41. doi: 10.1016/j.jbiotec.2013.08.012. Epub 2013 Aug 29.
The aim of this study is to investigate the effects of an artificially controlled environment, particularly elevated total pressure, on net photosynthesis and respiration during plant growth. Pressure directly affects not only cells and organelles in leaves but also the diffusion coefficients and degrees of solubility of CO2 and O2. In this study, the effects of elevated total pressure on the rates of net photosynthesis and respiration of a model plant, Arabidopsis thaliana, were investigated in a chamber that newly developed in this study to control the total pressure. The results clearly showed that the rate of respiration decreased linearly with increasing total pressure at a high humidity. The rate of respiration decreased linearly with increasing total pressure up to 0.2 MPa, and increased with increasing total pressure from 0.3 to 0.5 MPa at a low humidity. The rate of net photosynthesis decreased linearly with increasing total pressure under a constant partial pressure of CO2 at 40 Pa. On the other hand, the rate of net photosynthesis was clearly increased by up to 1.6-fold with increasing total pressure and partial pressure of CO2.
本研究旨在探讨人为控制环境，特别是升高总压对植物生长过程中净光合作用和呼吸作用的影响。压力不仅直接影响叶片中的细胞和细胞器，还影响 CO2 和 O2 的扩散系数和溶解度。在这项研究中，我们在一个新开发的腔室内研究了升高总压对拟南芥净光合作用和呼吸作用速率的影响，该腔室可控制总压。结果清楚地表明，在高湿度下，呼吸速率随总压的升高呈线性下降。呼吸速率随总压升高呈线性下降，在 0.2 MPa 以下，在低湿度下，呼吸速率随总压从 0.3 至 0.5 MPa 升高而升高。在 CO2 分压为 40 Pa 恒定的条件下，净光合作用速率随总压升高呈线性下降。另一方面，净光合作用速率随总压和 CO2 分压的升高呈明显增加，最高可达 1.6 倍。
