Wang B, Wang R R, Li J W, Ma Y L, Sheng W M, Li M F, Wang Q C
College of Horticulture, Northwest A and F University, Yangling, Shaanxi, China.
Cryo Letters. 2013 Jul-Aug;34(4):369-80.
China is now the largest potato producing country worldwide. The establishment of efficient conservation techniques for potato germplasm is a prerequisite for breeding of elite cultivars. Potato viral diseases have been a great threat for sustainable potato production in China. Use of virus-free seed tubers is an effective and practical means to control viral diseases. In the present study, three vitrification-based cryopreservation techniques, i.e. droplet-vitrification, encapsulation-vitrification and vitrification were successfully developed for the first time for China's potato. Cultivar 'Zihuabai' was used to optimize key parameters involved in the three vitrification-based procedures. With the optimized parameters, shoot regrowth percentages of 71%, 76% and 43% were obtained for droplet-vitrification, encapsulation-vitrification and vitrification, respectively. The three protocols developed were further tested with eight China's major cultivars, with average shoot regrowth of 61%, 38% and 28% for droplet-vitrification, encapsulation-vitrification and vitrification, respectively. Successful development of the three cryopreservation procedures using a single cultivar will facilitate a number of comparative studies such as cryo-injury, regrowth pattern, genetic stability and efficiency of virus elimination. Testing these three cryogenic procedures for potato major cultivars representing a wide range of genetic background, will help the establishment of potato cryobanking in China and the production of virus-free plants.
中国是目前全球最大的马铃薯生产国。建立高效的马铃薯种质保存技术是培育优良品种的前提条件。马铃薯病毒病一直是中国马铃薯可持续生产的巨大威胁。使用脱毒种薯是控制病毒病的有效且实用的手段。在本研究中，首次成功为中国马铃薯开发了三种基于玻璃化法的超低温保存技术，即滴冻玻璃化法、包埋玻璃化法和玻璃化法。利用品种“紫花白”对这三种基于玻璃化法的程序所涉及的关键参数进行了优化。采用优化后的参数，滴冻玻璃化法、包埋玻璃化法和玻璃化法的芽再生率分别达到了71%、76%和43%。所开发的这三种方法进一步用中国的八个主要品种进行了测试，滴冻玻璃化法、包埋玻璃化法和玻璃化法的平均芽再生率分别为61%、38%和28%。利用单一品种成功开发这三种超低温保存程序将有助于开展许多比较研究，如冷冻损伤、再生模式、遗传稳定性和病毒消除效率等。对代表广泛遗传背景的马铃薯主要品种测试这三种低温程序，将有助于在中国建立马铃薯超低温种质库并生产脱毒苗。
