Department of Botany, Faculty of Science, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India.
Environ Toxicol Pharmacol. 2013 Nov;36(3):875-82. doi: 10.1016/j.etap.2013.08.001. Epub 2013 Aug 11.
The present study is conducted to investigate the antioxidant potential of Jatropha curcas root bark extract (RB4 fraction) and leaf extract (L1 fraction), and to study their effects on UVB-radiation-induced DNA damage in cultured human blood lymphocytes. In this study, J. curcas showed strong antioxidant property in different free radical scavenging systems. Both the fractions effectively scavenged hydroxyl (OH), superoxide anion (O₂(·-)), 1,1-diphenyl-2-picrylhydrazyl (DPPH·) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid radical cation (ABTS(·+)) in a concentration-dependent manner. The IC₅₀ (Inhibitory Concentration 50) values of J. curcas fractions were compared to standard ascorbic acid used in this study. The antioxidant potential of a compound was directly proportional to the photoprotective effect. In this study, human peripheral blood lymphocytes (HPBL) were exposed to UVB-radiation and there was an increase in comet attributes (% tail DNA, tail length, tail movement and Olive tail moment). Jatropha curcas RB4 fraction and L1 fraction treatment before UVB-irradiation significantly decreased the % tail DNA, tail length, tail moment and Olive tail moment in irradiated HPBL. These results suggested that J. curcas exhibited strong antioxidant property and RB4 and L1 fractions protected UVB-radiation-induced DNA damage in HPBL.
本研究旨在探讨麻疯树根皮提取物（RB4 馏分）和叶提取物（L1 馏分）的抗氧化潜力，并研究它们对培养的人血淋巴细胞中 UVB 辐射诱导的 DNA 损伤的影响。在本研究中，麻疯树表现出很强的抗氧化特性，在不同的自由基清除系统中均有体现。这两个馏分都能有效清除羟基（OH）、超氧阴离子（O₂(·-)）、1,1-二苯基-2-苦基肼基（DPPH·）和 2,2-联氮-双-3-乙基苯并噻唑啉-6-磺酸自由基阳离子（ABTS(·+)），且具有浓度依赖性。与本研究中使用的标准抗坏血酸相比，麻疯树馏分的 IC₅₀（抑制浓度 50）值。化合物的抗氧化能力与光保护效果直接成正比。在本研究中，人外周血淋巴细胞（HPBL）暴露于 UVB 辐射下，彗星特征（%尾部 DNA、尾部长度、尾部运动和橄榄尾矩）增加。麻疯树 RB4 馏分和 L1 馏分在 UVB 照射前处理可显著降低辐照 HPBL 中的%尾部 DNA、尾部长度、尾部矩和橄榄尾矩。这些结果表明，麻疯树具有很强的抗氧化特性，RB4 和 L1 馏分可保护 HPBL 免受 UVB 辐射诱导的 DNA 损伤。
