Shahidul Islam, Yang Huaan, Yan Guijun
Faculty of Science, School of Plant Biology, The University of Western Australia, Crawley, WA, Australia.
Methods Mol Biol. 2013;1069:179-201. doi: 10.1007/978-1-62703-613-9_13.
Since the development of molecular markers to tag genes of agronomic traits of interests, molecular markers have played an increasingly significant role in breeding programs. Molecular markers have been implemented for large-scale marker-assisted selection in the breeding program of many important crops including lupin. So far, more than a dozen molecular markers for disease resistance genes and for other agronomic traits of interest have been developed in lupin. The DNA fingerprinting method, "MFLP" has played a pivotal role in the success of lupin breeding program in Australia. Here, we describe the MFLP technique used in lupin breeding which could be easily transferable to other crop species.
自从用于标记感兴趣的农艺性状基因的分子标记出现以来，分子标记在育种计划中发挥着越来越重要的作用。分子标记已被应用于包括羽扇豆在内的许多重要作物的育种计划中的大规模标记辅助选择。到目前为止，羽扇豆中已经开发出了十几种用于抗病基因和其他感兴趣的农艺性状的分子标记。“MFLP”DNA指纹图谱方法在澳大利亚羽扇豆育种计划的成功中发挥了关键作用。在这里，我们描述了羽扇豆育种中使用的MFLP技术，该技术可以很容易地转移到其他作物品种上。
