Field Helen P, Wheeler Michael J
Department of Specialist Laboratory Medicine, St. James's University Hospital, Leeds, UK.
Methods Mol Biol. 2013;1065:211-26. doi: 10.1007/978-1-62703-616-0_13.
A number of androgens are measured for clinical purposes. Most laboratories will run a testosterone assay but the requirement for other androgens may be too small for a laboratory to set up their own assay. In these cases samples would be sent to a specialized laboratory. In the routine laboratory testosterone is analyzed on automated systems but these lack the sensitivity to accurately measure the hormone in children and normal women. Many laboratories analyzed such samples by radioimmunoassay, but tandem mass spectrometry is now replacing these assays. This chapter provides simple methods for measuring the androgens in serum and saliva by radioimmunoassay and tandem mass spectrometry.
为了临床目的，会检测多种雄激素。大多数实验室会进行睾酮检测，但对其他雄激素的检测需求可能太少，以至于实验室无法自行开展检测。在这种情况下，样本会被送往专门的实验室。在常规实验室中，睾酮是在自动化系统上进行分析的，但这些系统缺乏准确测量儿童和正常女性体内该激素的灵敏度。许多实验室通过放射免疫分析法分析此类样本，但串联质谱法现在正在取代这些分析方法。本章提供了通过放射免疫分析法和串联质谱法测量血清和唾液中雄激素的简单方法。
