白细胞介素-13基因多态性及其与巴基斯坦患者特应性哮喘和鼻炎的关联。

IL-13 gene polymorphisms and their association with atopic asthma and rhinitis in Pakistani patients.

作者信息

Shazia Micheal, Kanza Minhas, Mehwish Ishaque, Irum Shabbir, Farida Ahmed, Asifa Ahmed

机构信息

Department of Biosciences, COMSATS Institute of Information Technology, Islamabad-44000, Pakistan and Department of Ophthalmology, Radboud University Nijmegen Medical Centre; Nijmegen, Netherlands.

出版信息

Iran J Allergy Asthma Immunol. 2013 Aug 28;12(4):391-6.

PMID:23996716

Abstract

Interleukin-13 (IL-13) is known to be a key regulator in immunoglobulin E (IgE) synthesis, mucus hypersecretion and airway hyperresponsiveness. Single nucleotide polymorphisms in IL-13 are associated with allergic phenotypes in several ethnically diverse populations.This study was performed in 214 atopic patients (asthma n=108, allergic rhinitis n=106) and sex-matched healthy controls (n=120). Genotyping of IL-13 gene polymorphisms was performed using polymerase chain reaction-based restriction fragment length polymorphism method.A statistically significant association of the A-1512C polymorphism in IL13 gene was observed with atopy (p<0.001; χ2=19.0). Upon stratification of the data into asthma and AR association was revealed with both asthma (p=0.01; χ2=8.80) and AR (p<0.001; χ2=24.3) in Pakistani patients. Higher odds ratio (OR 95% CI) was observed for AR 3.42 (2.04-5.76) relative to asthma 2.40 (1.41-4.09) for the C allele compared to controls.In conclusion the study provides the evidence A-1512C polymorphisms in IL-13 is a risk factor for asthma and AR.

摘要

白细胞介素-13（IL-13）是免疫球蛋白E（IgE）合成、黏液分泌过多和气道高反应性的关键调节因子。IL-13中的单核苷酸多态性与多个不同种族人群的过敏表型相关。本研究纳入了214例特应性患者（哮喘患者108例，变应性鼻炎患者106例）以及性别匹配的健康对照者（120例）。采用基于聚合酶链反应的限制性片段长度多态性方法对IL-13基因多态性进行基因分型。观察到IL13基因A-1512C多态性与特应性显著相关（p<0.001；χ2=19.0）。将数据按哮喘和变应性鼻炎分层后发现，在巴基斯坦患者中，该多态性与哮喘（p=0.01；χ2=8.80）和变应性鼻炎（p<0.001；χ2=24.3）均相关。与对照组相比，C等位基因在变应性鼻炎中的优势比（OR 95%CI）为3.42（2.04 - 5.76），高于哮喘的2.40（1.41 - 4.09）。总之，本研究提供了证据表明IL-13的A-1512C多态性是哮喘和变应性鼻炎的危险因素。