中国汉族人群中ADAM33基因多态性与成人过敏性哮喘及鼻炎的相关性
Association of ADAM33 gene polymorphisms with adult allergic asthma and rhinitis in a Chinese Han population.
作者信息
Su Dongju, Zhang Ximei, Sui Hong, Lü Fuzhen, Jin Lianhong, Zhang Jing
机构信息
Department of Respiratory, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, PR China.
出版信息
BMC Med Genet. 2008 Sep 9;9:82. doi: 10.1186/1471-2350-9-82.
BACKGROUND
Rhinitis and asthma are very common diseases involving genetic and environmental factors. Most patients with asthma also have rhinitis, which suggests the concept of 'one airway, one disease.' A disintegrin and metalloproteinase 33 (ADAM33) is the first asthma-susceptible gene to be discovered by positional cloning. To evaluate the potential influence of ADAM33 gene polymorphisms on allergic rhinitis (AR) and allergic asthma (AS), a case-control study was conducted on the Han population of northeast China.
METHODS
Six polymorphic sites (V4, T+1, T2, T1, S1, and Q-1) were genotyped in 128 patients with AR, 181 patients with AS, and 151 healthy controls (CTR). Genotypes were determined by the polymerase chain restriction fragment length polymorphism (PCR-RFLP) method. Data were analyzed using the chi-square test with Haploview software.
RESULTS
The single nucleotide polymorphisms (SNPs), V4 G/C, T+1 A/G, and T1 G/A, of the ADAM33 gene may be the causal variants in AR, whereas ADAM33 V4 G/C, T2 A/G, T1 G/A, and Q-1A/G may participate in the susceptibility of AS.
CONCLUSION
These results suggest that polymorphisms of the ADAM33 gene may modify individual susceptibility to AR and AS in a Chinese Han population.
背景
鼻炎和哮喘是涉及遗传和环境因素的常见疾病。大多数哮喘患者也患有鼻炎，这提示了“一个气道，一种疾病”的概念。解整合素金属蛋白酶33（ADAM33）是首个通过定位克隆发现的哮喘易感基因。为评估ADAM33基因多态性对变应性鼻炎（AR）和变应性哮喘（AS）的潜在影响，对中国东北地区汉族人群进行了一项病例对照研究。
方法
对128例AR患者、181例AS患者和151例健康对照（CTR）的6个多态性位点（V4、T +1、T2、T1、S1和Q -1）进行基因分型。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法确定基因型。使用Haploview软件通过卡方检验分析数据。
结果
ADAM33基因的单核苷酸多态性（SNP），即V4 G/C、T +1 A/G和T1 G/A，可能是AR的致病变异，而ADAM33 V4 G/C、T2 A/G、T1 G/A和Q -1A/G可能参与AS的易感性。
结论
这些结果表明，ADAM33基因多态性可能改变中国汉族人群对AR和AS的个体易感性。
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