Su Dongju, Zhang Ximei, Sui Hong, Lü Fuzhen, Jin Lianhong, Zhang Jing
Department of Respiratory, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, PR China.
BMC Med Genet. 2008 Sep 9;9:82. doi: 10.1186/1471-2350-9-82.
Rhinitis and asthma are very common diseases involving genetic and environmental factors. Most patients with asthma also have rhinitis, which suggests the concept of 'one airway, one disease.' A disintegrin and metalloproteinase 33 (ADAM33) is the first asthma-susceptible gene to be discovered by positional cloning. To evaluate the potential influence of ADAM33 gene polymorphisms on allergic rhinitis (AR) and allergic asthma (AS), a case-control study was conducted on the Han population of northeast China.
Six polymorphic sites (V4, T+1, T2, T1, S1, and Q-1) were genotyped in 128 patients with AR, 181 patients with AS, and 151 healthy controls (CTR). Genotypes were determined by the polymerase chain restriction fragment length polymorphism (PCR-RFLP) method. Data were analyzed using the chi-square test with Haploview software.
The single nucleotide polymorphisms (SNPs), V4 G/C, T+1 A/G, and T1 G/A, of the ADAM33 gene may be the causal variants in AR, whereas ADAM33 V4 G/C, T2 A/G, T1 G/A, and Q-1A/G may participate in the susceptibility of AS.
These results suggest that polymorphisms of the ADAM33 gene may modify individual susceptibility to AR and AS in a Chinese Han population.
鼻炎和哮喘是涉及遗传和环境因素的常见疾病。大多数哮喘患者也患有鼻炎，这提示了“一个气道，一种疾病”的概念。解整合素金属蛋白酶33（ADAM33）是首个通过定位克隆发现的哮喘易感基因。为评估ADAM33基因多态性对变应性鼻炎（AR）和变应性哮喘（AS）的潜在影响，对中国东北地区汉族人群进行了一项病例对照研究。
对128例AR患者、181例AS患者和151例健康对照（CTR）的6个多态性位点（V4、T +1、T2、T1、S1和Q -1）进行基因分型。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法确定基因型。使用Haploview软件通过卡方检验分析数据。
ADAM33基因的单核苷酸多态性（SNP），即V4 G/C、T +1 A/G和T1 G/A，可能是AR的致病变异，而ADAM33 V4 G/C、T2 A/G、T1 G/A和Q -1A/G可能参与AS的易感性。
这些结果表明，ADAM33基因多态性可能改变中国汉族人群对AR和AS的个体易感性。
