自愿运动促进成年大鼠下丘脑祖细胞的增殖和分化。
Voluntary exercise promotes proliferation and differentiation of adult rat hypothalamus progenitor cells.
作者信息
Li Guanghua, Matsuzki Kentaro, Wang Yin, Zhao Nan, Yang Min, Shido Osamu
机构信息
Department of Physiology, Ningxia Medical University, Yinchuan 750004, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Aug;33(8):1099-106.
OBJECTIVE
To investigate the effect of voluntary exercise on the proliferation and differentiation of hypothalamus progenitor cells in adult rats.
METHODS
Male Wistar rats were divided into voluntary exercise (EX) and sedentary (SE) groups, both of which were further divided into 6 subgroups for observation on days 6, 13, 23, 33, 43 and 53. Bromodeoxyuridine (BrdU) was intraperitoneally injected daily for 5 consecutive days after commencing voluntary exercise, and at the specified time points during voluntary exercise, the rats' brains were removed to observe the numbers of BrdU-positive cells in the hypothalamus.
RESULTS
Immunohistochemical analysis showed that the numbers of BrdU-positive cells in the hypothalamus of EX subgroups were significantly greater than those of SE subgroups on days 23, 33, 43 and 53. In EX group, the number of BrdU-positive cells double-stained for a mature neuron marker increased after 43 days of voluntary exercise, which did not occur in SE group.
CONCLUSION
Long-term voluntary exercise can promote the proliferation of neuronal progenitor cells in the hypothalamus and their differentiation into neurons.
目的
研究自愿运动对成年大鼠下丘脑祖细胞增殖和分化的影响。
方法
将雄性Wistar大鼠分为自愿运动组（EX）和久坐组（SE），两组均进一步分为6个亚组，分别在第6、13、23、33、43和53天进行观察。在开始自愿运动后连续5天每天腹腔注射溴脱氧尿苷（BrdU），并在自愿运动期间的指定时间点取出大鼠大脑，观察下丘脑BrdU阳性细胞的数量。
结果
免疫组织化学分析显示，在第23、33、43和53天，EX亚组下丘脑BrdU阳性细胞数量显著多于SE亚组。在EX组中，自愿运动43天后，双染成熟神经元标志物的BrdU阳性细胞数量增加，而SE组未出现这种情况。
结论
长期自愿运动可促进下丘脑神经元祖细胞的增殖及其向神经元的分化。
Zotero 插件