Bakouche O, Lachman L B
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston.
Yale J Biol Med. 1990 Mar-Apr;63(2):95-107.
Liposomes containing membrane IL-1, Iak, and the antigen conalbumin were evaluated as "synthetic antigen presenting cells." The role of these three molecules in macrophage-T cell interaction was studied by testing their ability to induce the proliferation of a T-cell clone specific to conalbumin (the D10 cell line) or immune spleen cells sensitized three times in vivo with conalbumin. In the latter case, splenic macrophages were eliminated by adherence and a lysomotropic agent. The antigen conalbumin was presented on the surface of the liposomes as native undigested protein. When the liposomes presented native conalbumin, Iak, and membrane IL-1, significant proliferation occurred, but if the liposomes lacked membrane IL-1, the proliferation of the T-cell clone and the spleen cells reached only about 60 percent of the previous signal. Native conalbumin and class II antigen alone were required for T-cell activation, while membrane IL-1 only amplified the response. When the liposomes were made with only Iak and membrane IL-1, lacking conalbumin, there was no proliferation of antigen-specific target cells. These results indicated that in this synthetic system, membrane IL-1 increases the magnitude of the response but is not essential for the proliferative response of antigen-specific T cells.
含有膜白细胞介素-1（IL-1）、白细胞相关抗原（Iak）和抗原伴清蛋白的脂质体被评估为“合成抗原呈递细胞”。通过测试它们诱导对伴清蛋白特异的T细胞克隆（D10细胞系）或体内用伴清蛋白致敏三次的免疫脾细胞增殖的能力，研究了这三种分子在巨噬细胞-T细胞相互作用中的作用。在后一种情况下，通过贴壁和溶酶体亲和剂消除脾巨噬细胞。抗原伴清蛋白以天然未消化蛋白的形式呈现在脂质体表面。当脂质体呈现天然伴清蛋白、Iak和膜IL-1时，发生显著增殖，但如果脂质体缺乏膜IL-1，T细胞克隆和脾细胞的增殖仅达到先前信号的约60%。单独的天然伴清蛋白和II类抗原是T细胞活化所必需的，而膜IL-1仅增强反应。当脂质体仅由Iak和膜IL-1制成而缺乏伴清蛋白时，抗原特异性靶细胞没有增殖。这些结果表明，在这个合成系统中，膜IL-1增加反应的强度，但对抗原特异性T细胞的增殖反应不是必需的。
