大鼠皮肤组织蛋白酶D的纯化及生化特性研究

Purification and biochemical characterization of rat skin cathepsin D.

作者信息

Heikkinen J E, Järvinen M, Jansén C R

出版信息

J Invest Dermatol. 1975 Sep;65(3):272-8. doi: 10.1111/1523-1747.ep12598339.

DOI:10.1111/1523-1747.ep12598339

Abstract

The hemoglobin-hydrolyzing, acidic proteinase activity of rat skin was purified by using ammonium sulfate precipitation. Sephadex G-100 gel column chromatography, acid treatment, and DEAE-cellulose column chromatography, giving a purification coefficient of 182. The pH optimum, molecular size, substrate specificity, as well as inhibitor and activator sensitivity of the enzyme preparation, corresponded closely to those of cathepsin D. The enzyme activity was separated from cathepsin B1. The present status of the knowledge of skin cathespins is reviewed.

摘要

通过硫酸铵沉淀、Sephadex G - 100凝胶柱色谱、酸处理和DEAE - 纤维素柱色谱法，对大鼠皮肤的血红蛋白水解酸性蛋白酶活性进行了纯化，纯化系数为182。该酶制剂的最适pH、分子大小、底物特异性以及对抑制剂和激活剂的敏感性与组织蛋白酶D密切相关。该酶活性与组织蛋白酶B1分离。本文综述了皮肤组织蛋白酶的知识现状。

相似文献

1

Purification and biochemical characterization of rat skin cathepsin D.大鼠皮肤组织蛋白酶D的纯化及生化特性研究

J Invest Dermatol. 1975 Sep;65(3):272-8. doi: 10.1111/1523-1747.ep12598339.

2

alpha-N-benzoylarginine-2-naphthylamide hydrolase (cathepsin B1 ?) from rat skin. II. Purification of the enzyme and demonstration of two inhibitors in the skin.来自大鼠皮肤的α-N-苯甲酰精氨酸-2-萘酰胺水解酶（组织蛋白酶B1？）。II. 酶的纯化及皮肤中两种抑制剂的证明。

Acta Chem Scand B. 1975;29(7):772-80. doi: 10.3891/acta.chem.scand.29b-0772.

3

Human skin proteases. Separation and characterization of two acid proteases resembling cathepsin B1 and cathepsin D and of an inhibitor of cathepsin B1.人类皮肤蛋白酶。两种类似于组织蛋白酶B1和组织蛋白酶D的酸性蛋白酶以及组织蛋白酶B1抑制剂的分离与特性研究

Arch Dermatol Res (1975). 1976 Jun 21;255(3):317-30. doi: 10.1007/BF00561502.

4

Human cathepsin B1. Purification and some properties of the enzyme.人组织蛋白酶B1。该酶的纯化及某些特性。

Biochem J. 1973 Apr;131(4):809-22. doi: 10.1042/bj1310809.

5

Alpha-N-benzoylarginine-2-naphthylamide hydrolase (cathepsin B1?) from rat skin. I. Preliminary experiments with skin extract.来自大鼠皮肤的α-N-苯甲酰精氨酸-2-萘酰胺水解酶（组织蛋白酶B1？）。I. 皮肤提取物的初步实验。

Acta Chem Scand B. 1975;29(6):671-6. doi: 10.3891/acta.chem.scand.29b-0671.

6

The structure and function of acid proteases. VIII. Purification and characterization of cathepsins D from Japanese monkey lung.酸性蛋白酶的结构与功能。VIII. 日本猕猴肺组织中组织蛋白酶D的纯化与特性分析

J Biochem. 1978 Feb;83(2):441-51. doi: 10.1093/oxfordjournals.jbchem.a131931.

7

Isolation and characterization of cathepsin B from bovine brain.牛脑组织中组织蛋白酶B的分离与鉴定

Neurochem Res. 1986 Jun;11(6):851-67. doi: 10.1007/BF00965209.

8

Alpha-N-Benzoylarginine-2-naphthylamide hydrolase (cathepsin BI?) from rat skin. III. Substrate specificity, modifier characteristics, and transformation of the enzyme at acidic pH.来自大鼠皮肤的α-N-苯甲酰精氨酸-2-萘酰胺水解酶（组织蛋白酶BI？）。III. 底物特异性、修饰剂特性以及该酶在酸性pH下的转变

Acta Chem Scand B. 1976;30(1):53-60. doi: 10.3891/acta.chem.scand.30b-0053.

9

The purification of bovine cathepsin B1 and its mode of action on bovine collagens.牛组织蛋白酶B1的纯化及其对牛胶原蛋白的作用方式。

Biochem J. 1974 Mar;137(3):547-57. doi: 10.1042/bj1370547.

10

Rat kidney renin and cathepsin D: purification and comparison of properties.大鼠肾脏肾素与组织蛋白酶D：特性的纯化与比较

Biochemistry. 1983 Nov 22;22(24):5476-81. doi: 10.1021/bi00293a004.

引用本文的文献

1

Human skin proteases. Separation and characterization of two acid proteases resembling cathepsin B1 and cathepsin D and of an inhibitor of cathepsin B1.人类皮肤蛋白酶。两种类似于组织蛋白酶B1和组织蛋白酶D的酸性蛋白酶以及组织蛋白酶B1抑制剂的分离与特性研究

Arch Dermatol Res (1975). 1976 Jun 21;255(3):317-30. doi: 10.1007/BF00561502.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。