Nagata Maria J H, de Campos Natália, Messora Michel R, Pola Natália M, Santinoni Carolina S, Bomfim Suely R M, Fucini Stephen E, Ervolino Edilson, de Almeida Juliano M, Theodoro Letícia H, Garcia Valdir G
Department of Surgery and Integrated Clinic, Dental School of Araçatuba, UNESP - São Paulo State University, Araçatuba, São Paulo, Brazil.
J Periodontol. 2014 Jun;85(6):770-8. doi: 10.1902/jop.2013.130318. Epub 2013 Sep 3.
This study histomorphometrically analyzes the influence of platelet-rich plasma (PRP), low-level laser therapy (LLLT), or their combination on the healing of periodontal fenestration defects (PFDs) in rats.
PFDs were surgically created in the mandibles of 80 rats. The animals were randomly divided into four groups: 1) C (control) and 2) PRP, defects were filled with blood clot or PRP, respectively; 3) LLLT and 4) PRP/LLLT, defects received laser irradiation, were filled with blood clot or PRP, respectively, and then irradiated again. Animals were euthanized at either 10 or 30 days post-surgery. Percentage of new bone (NB), density of newly formed bone (DNB), new cementum (NC), and extension of remaining defect (ERD) were histomorphometrically evaluated. Data were statistically analyzed (analysis of variance; Tukey test, P <0.05).
At 10 days, group PRP presented ERD significantly lower than group C. At 30 days, group PRP presented NB and DNB significantly greater than group C. Groups LLLT, PRP, and PRP/LLLT showed significant NC formation at 30 days, with collagen fibers inserted obliquely or perpendicularly to the root surface. NC formation was not observed in any group C specimen.
LLLT, PRP, or their combination all promoted NC formation with a functional periodontal ligament. The combination PRP/LLLT did not show additional positive effects compared to the use of either therapy alone.
本研究通过组织形态计量学分析富血小板血浆（PRP）、低强度激光治疗（LLLT）或二者联合应用对大鼠牙周开窗缺损（PFD）愈合的影响。
在80只大鼠的下颌骨上手术制造PFD。动物被随机分为四组：1）C组（对照组）和2）PRP组，缺损分别用血凝块或PRP填充；3）LLLT组和4）PRP/LLLT组，缺损接受激光照射，分别用血凝块或PRP填充，然后再次照射。在术后10天或30天对动物实施安乐死。通过组织形态计量学评估新骨（NB）百分比、新形成骨的密度（DNB）、新牙骨质（NC）以及剩余缺损的扩展（ERD）。对数据进行统计学分析（方差分析；Tukey检验，P<0.05）。
在术后10天，PRP组的ERD显著低于C组。在术后30天，PRP组的NB和DNB显著高于C组。LLLT组、PRP组和PRP/LLLT组在术后30天均显示出显著的NC形成，胶原纤维倾斜或垂直于牙根表面插入。在任何C组标本中均未观察到NC形成。
LLLT、PRP或二者联合应用均促进了具有功能性牙周韧带的NC形成。与单独使用任何一种治疗方法相比，PRP/LLLT联合应用未显示出额外的积极效果。
