胡子鲶和三线胡鲶特定免疫反应的季节性变化。

Seasonal changes in selected immune response of Mystus gulio and Mystus vittatus.

作者信息

Sakthivel M, Deivasigamani B, Alagappan K M, Kumaran S, Balamurugan S, Rajasekar T

机构信息

Faculty of Marine Sciences, Annamalai University, Parangipettai-608 502, India.

出版信息

J Environ Biol. 2013 Jan;34(1):37-42.

PMID:24006805

Abstract

Estuaries are considered as highly potential area for that including feeding, spawning and nursery rearing of most of the finfishes and shellfishes. In the present investigation, two species of catfish (M. gulio and M. vittatus) were selected to study the impact of season on their immune organs (kidney and spleen) and selected immune response from Vellar estuary. The physico-chemical parameters (pH, temperature, salinity and rainfall) were measured during the sampling period 2009-10. The non-specific immune parameters (WBC count, lysozyme activity and NBT assay) were analyzed. The immune organs (head-kidney and spleen) variation was also observed by histological studies. Our results stated that the WBCs count of M. gulio and M. vittatus increased during summer (10.3 and 10.1 x 10(6) ml(-1)) season and decreased in monsoon (6.8 and 7.0 x 10(6) ml(-1)). In contrast, lysozyme activity was highest in post monsoon (1540 and 1525 U min(-1) ml(-1)) and lowest in summer (1000 and 960 U min(-1) ml(-1)). The activity of NBT was highest in monsoon (0.68 and 0.65 at 540 nm) and lowest in summer (0.012 and 0.2 at 540 nm). The histological observation implies that the cell variations were different in respect to different seasons.

摘要

河口被认为是极具潜力的区域，适合大多数硬骨鱼和贝类的觅食、产卵和幼鱼培育。在本研究中，选取了两种鲶鱼（吉利巴沙鱼和条纹鲶鱼）来研究季节对其免疫器官（肾脏和脾脏）的影响，并从韦拉尔河口选取了免疫反应进行研究。在2009 - 2010年采样期间测量了理化参数（pH值、温度、盐度和降雨量）。分析了非特异性免疫参数（白细胞计数、溶菌酶活性和NBT检测）。还通过组织学研究观察了免疫器官（头肾和脾脏）的变化。我们的结果表明，吉利巴沙鱼和条纹鲶鱼的白细胞计数在夏季（分别为10.3和10.1×10⁶ ml⁻¹）增加，在季风季节（分别为6.8和7.0×10⁶ ml⁻¹）减少。相比之下，溶菌酶活性在季风后最高（分别为1540和1525 U min⁻¹ ml⁻¹），在夏季最低（分别为1000和960 U min⁻¹ ml⁻¹）。NBT的活性在季风季节最高（在540 nm处分别为0.68和0.65），在夏季最低（在540 nm处分别为0.012和0.2）。组织学观察表明，不同季节细胞变化不同。