Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry, Campinas State University, Piracicaba, SP, Brazil.
J Proteomics. 2013 Oct 8;91:544-55. doi: 10.1016/j.jprot.2013.08.016. Epub 2013 Sep 2.
Dental cementum (DC) is a bone-like tissue covering the tooth root and responsible for attaching the tooth to the alveolar bone (AB) via the periodontal ligament (PDL). Studies have unsuccessfully tried to identify factors specific to DC versus AB, in an effort to better understand DC development and regeneration. The present study aimed to use matched human DC and AB samples (n=7) to generate their proteomes for comparative analysis. Bone samples were harvested from tooth extraction sites, whereas DC samples were obtained from the apical root portion of extracted third molars. Samples were denatured, followed by protein extraction reduction, alkylation and digestion for analysis by nanoAcquity HPLC system and LTQ-FT Ultra. Data analysis demonstrated that a total of 318 proteins were identified in AB and DC. In addition to shared proteins between these tissues, 105 and 83 proteins exclusive to AB or DC were identified, respectively. This is the first report analyzing the proteomic composition of human DC matrix and identifying putative unique and enriched proteins in comparison to alveolar bone. These findings may provide novel insights into developmental differences between DC and AB, and identify candidate biomarkers that may lead to more efficient and predictable therapies for periodontal regeneration.
Periodontal disease is a highly prevalent disease affecting the world population, which involves breakdown of the tooth supporting tissues, the periodontal ligament, alveolar bone, and dental cementum. The lack of knowledge on specific factors that differentiate alveolar bone and dental cementum limits the development of more efficient and predictable reconstructive therapies. In order to better understand cementum development and potentially identify factors to improve therapeutic outcomes, we took the unique approach of using matched patient samples of dental cementum and alveolar bone to generate and compare a proteome list for each tissue. A potential biomarker for dental cementum was identified, superoxide dismutase 3 (SOD3), which is found in cementum and cementum-associated cells in mouse, pig, and human tissues. These findings may provide novel insights into developmental differences between alveolar bone and dental cementum, and represent the basis for improved and more predictable therapies.
牙骨质（DC）是一种覆盖牙根的骨样组织，通过牙周韧带（PDL）将牙齿附着在牙槽骨（AB）上。研究未能成功地确定 DC 与 AB 特有的因素，以更好地了解 DC 的发育和再生。本研究旨在使用匹配的人 DC 和 AB 样本（n=7）生成它们的蛋白质组进行比较分析。骨样本取自拔牙部位，而 DC 样本取自第三磨牙根尖部分的牙骨质。对样本进行变性处理，然后进行蛋白质提取还原、烷基化和消化，以便通过 nanoAcquity HPLC 系统和 LTQ-FT Ultra 进行分析。数据分析表明，AB 和 DC 中共鉴定出 318 种蛋白质。除了这些组织共有的蛋白质外，还分别鉴定出 105 种和 83 种 AB 或 DC 特有的蛋白质。这是首次分析人牙骨质基质的蛋白质组组成，并鉴定出与牙槽骨相比具有独特性和丰富性的蛋白质。这些发现可能为 DC 和 AB 之间的发育差异提供新的见解，并确定可能导致牙周再生更有效和可预测的治疗的候选生物标志物。
牙周病是一种影响世界人口的高发疾病，涉及牙齿支持组织、牙周韧带、牙槽骨和牙骨质的破坏。对区分牙槽骨和牙骨质的特定因素的了解不足限制了更有效和可预测的重建治疗的发展。为了更好地了解牙骨质的发育并可能确定改善治疗效果的因素，我们采取了独特的方法，使用匹配的患者牙骨质和牙槽骨样本生成并比较每种组织的蛋白质组列表。鉴定出一种潜在的牙骨质生物标志物，即超氧化物歧化酶 3（SOD3），它存在于小鼠、猪和人组织中的牙骨质和牙骨质相关细胞中。这些发现可能为牙槽骨和牙骨质之间的发育差异提供新的见解，并为改善和更可预测的治疗提供基础。
