Friberg J, Gemzell C
Int J Fertil. 1977;22(3):148-54.
A comparison between freezing of human sperm using glycerol or glycerol egg-yolk citrate is presented. Freezing was performed with a gradual lowering of the temperature using alchol-liquid nitrogen or by immersion into liquid nitrogen. Great variations were observed within the same donor sperm sample when the sperm was frozen according to different techniques and marked post-thaw, motility variations were also found between spermatozoa from different donors. No statistically significant differences were found in the number of pregnancies obtained following inseminations with sperm frozen according to the two methods.
本文对使用甘油或甘油 - 蛋黄柠檬酸盐冷冻人类精子进行了比较。冷冻过程通过酒精 - 液氮逐步降温或浸入液氮来完成。当按照不同技术冷冻同一供体的精子样本时，观察到了很大差异，解冻后也发现不同供体的精子在活力上存在明显差异。两种方法冷冻的精子授精后获得的妊娠数量没有统计学上的显著差异。
