Onishi K, Kamida T, Momii Y, Abe T, Fujiki M
Department of Neurosurgery, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Oita, 879-5593, Japan.
Endocrine. 2014 May;46(1):154-9. doi: 10.1007/s12020-013-0046-4. Epub 2013 Sep 6.
The purpose of the present study was to define the clinical and pathological significance of nitric oxide synthase (NOS) in human pituitary adenomas, and to compare these values with those of the MIB-1 labeling index (LI) using an immunohistochemical method. Tissue specimens from 82 cases of surgically-treated pituitary adenomas were immunostained for hormone production for the MIB-1 LI and for the three NOS isoenzymes and five normal pituitary glands were immunostained for the three NOS isoenzymes as a control. The correlation between the clinical variables (age, functional status, tumor size, Hardy's grading, cavernous and/or sphenoid invasiveness, and progression) and mean MIB-1 LI, or between the same clinical variables and NOS immunoreactivity (IR) were analyzed. There was a statistically significant difference in the MIB-1 LI between macroadenomas and microadenomas, and between invasive adenomas and noninvasive adenomas. On the other hand, there was a statistically significant difference in the inducible NOS (iNOS) IR between invasive adenomas and noninvasive adenomas. Furthermore, the iNOS IR had a significant correlation with the MIB-1 LI. Invasive adenomas have a higher iNOS IR, and this correlated with the MIB-1 LI. These findings may be due to the function of iNOS, which plays an important role in tissue injury and repair.
本研究的目的是确定一氧化氮合酶（NOS）在人垂体腺瘤中的临床和病理意义，并使用免疫组织化学方法将这些值与MIB-1标记指数（LI）的值进行比较。对82例手术治疗的垂体腺瘤组织标本进行免疫染色，以检测激素分泌、MIB-1 LI以及三种NOS同工酶，另外对五个正常垂体进行免疫染色作为对照，以检测三种NOS同工酶。分析临床变量（年龄、功能状态、肿瘤大小、Hardy分级、海绵窦和/或蝶窦侵犯情况以及进展情况）与平均MIB-1 LI之间，或相同临床变量与NOS免疫反应性（IR）之间的相关性。大腺瘤与微腺瘤之间、侵袭性腺瘤与非侵袭性腺瘤之间的MIB-1 LI存在统计学显著差异。另一方面，侵袭性腺瘤与非侵袭性腺瘤之间的诱导型NOS（iNOS）IR存在统计学显著差异。此外，iNOS IR与MIB-1 LI具有显著相关性。侵袭性腺瘤具有较高的iNOS IR，且这与MIB-1 LI相关。这些发现可能归因于iNOS的功能，其在组织损伤和修复中起重要作用。
