Whittington M A, Little H J
Pharmacology Department, Medical School, University of Bristol, U.K.
Brain Res. 1990 Jul 23;523(2):237-44. doi: 10.1016/0006-8993(90)91492-y.
Extracellular recordings were made from isolated hippocampal slices, CA1 area, following withdrawal from chronic ethanol administration to mice of the C57 strain. The field potentials were followed for 7 h from preparation of the slices, in the absence of ethanol. Paired pulse potentiation was increased, and paired pulse inhibition decreased, in slices from ethanol-treated mice during the first four hours of the recording period. Orthodromic thresholds for elicitation of single and multiple population spikes were decreased by the ethanol treatment, in the later part of the recording period. The input/output curves for population spike area and population excitatory postsynaptic potential slope showed a shift to the left for the slices from ethanol-treated animals, but no change in the maximal response. Antidromic stimulation also demonstrated decreases in thresholds for single and multiple population spikes in tissues from ethanol-treated animals, during the later half of the recording period. The results indicate that there are several mechanisms by which neuronal excitability increases on withdrawal from chronic ethanol treatment. The changes follow different time courses and suggest multiple mechanisms underlying the behavioural signs seen during the ethanol withdrawal syndrome.
从C57品系小鼠慢性乙醇给药撤药后，对分离的海马切片CA1区进行细胞外记录。在无乙醇的情况下，从切片制备开始对场电位进行7小时的跟踪。在记录期的前四个小时，乙醇处理小鼠的切片中，双脉冲增强增加，双脉冲抑制减少。在记录期的后期，乙醇处理降低了诱发单个和多个群体锋电位的顺向阈值。群体锋电位面积和群体兴奋性突触后电位斜率的输入/输出曲线显示，乙醇处理动物的切片向左偏移，但最大反应无变化。在记录期的后半段，逆向刺激也显示乙醇处理动物组织中单个和多个群体锋电位的阈值降低。结果表明，慢性乙醇处理撤药后神经元兴奋性增加有多种机制。这些变化遵循不同的时间进程，提示乙醇戒断综合征期间出现的行为体征有多种潜在机制。
