Liu Chen, LV Xiao Tian, Zhu Wen Xue, QU Hao Yang, Gao Yong Xin, Guo Bao Yuan, Wang Hui Li
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, China.
Chirality. 2013 Dec;25(12):917-22. doi: 10.1002/chir.22234. Epub 2013 Sep 6.
The enantioselective bioaccumulation of diniconazole in Tenebrio molitor Linne larva was investigated with liquid chromatography tandem mass spectrometry based on the ChiralcelOD-3R[cellulose tri-(3,5-dimethylphenyl carbamate)] column. In this study we documented the effects of dietary supplementation with wheat bran contaminated by racemic diniconazole at two dose levels of 20 mg kg(-1) and 2 mg kg(-1) (dry weight) in Tenebrio molitor. The results showed that both doses of diniconazole were taken up by Tenebrio molitor rapidly in the first few days, the concentrations of R-enantiomer and S-enantiomer at high doses reached the highest level of 0.55 mg kg(-1) and 0.48 mg kg(-1) , respectively, on the 1(st) d, and the concentrations of them obtained a maxima of 0.129 mg kg(-1) and 0.128 mg kg(-1) at low dose, respectively, on the 3(rd) d, which means that the concentration of diniconazole was proportional to the time of achieving the highest accumulated level. It afterwards attained equilibrium after a sharp decline at both 20 mg kg(-1) and 2 mg kg(-1) of diniconazole. The determination results from the feces of Tenebrio molitor demonstrated that the extraction recovery (ER) values of the high dose group were higher than that of the low dose group and the values were all above 1; therefore, it could be inferred that enantiomerization existed in Tenebrio molitor. Additionally, the biota accumulation factor was used to evaluate the bioaccumulation of diniconazole enantiomers, showing that the bioaccumulation of diniconazole in Tenebrio molitor was enantioselective with preferential accumulation of S-enantiomer.
基于Chiralcel OD - 3R[三（3,5 - 二甲基苯基氨基甲酸酯）纤维素]柱，采用液相色谱串联质谱法研究了烯唑醇在黄粉虫幼虫体内的对映体选择性生物累积。在本研究中，我们记录了用外消旋烯唑醇污染的麦麸以20 mg kg(-1)和2 mg kg(-1)（干重）两种剂量水平对黄粉虫进行膳食补充的影响。结果表明，两种剂量的烯唑醇在最初几天内均被黄粉虫迅速吸收，高剂量下R - 对映体和S - 对映体的浓度分别在第1天达到最高水平0.55 mg kg(-1)和0.48 mg kg(-1)，低剂量下它们的浓度分别在第3天达到最大值0.129 mg kg(-1)和0.128 mg kg(-1)，这意味着烯唑醇的浓度与达到最高累积水平的时间成正比。之后，在20 mg kg(-1)和2 mg kg(-1)的烯唑醇处理下，其浓度在急剧下降后达到平衡。黄粉虫粪便的测定结果表明，高剂量组的提取回收率（ER）值高于低剂量组，且所有值均高于1；因此，可以推断黄粉虫体内存在对映体转化。此外用生物累积因子评估烯唑醇对映体的生物累积，结果表明烯唑醇在黄粉虫体内的生物累积具有对映体选择性，S - 对映体优先累积。
