荆芥挥发油及其主要成分抗流感病毒的作用及机制研究

[Study on effect and mechanism of volatile oil of schizonepetae herba and its essential components against influenza virus].

作者信息

He Ting, Tang Qi, Zeng Nan, Gou Ling, Liu Jin-Wei, Yang Jing, Yu Liu, Wang Zhe, Gong Xi-Ping

机构信息

Cultivation Base of State and Provincial Key Laboratory for Research, Development and Utilization of Traditional Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2013 Jun;38(11):1772-7.

PMID:24010294

Abstract

OBJECTIVE

To observe the effect of volatile oil of Schizonepetae Herba (VOSH), and its essential components-menthone and pulegone against anti-influenza virus A/PR/8/34 (H1N1) in vivo and in vitro, as well as the signaling mechanism of its toll-like receptor/interferon (TLR/IFN).

METHOD

The lung-adapted PR-8 virus model was prepared in mice. They were administered with preventive and therapeutic drugs, and the hemagglutination titer of model animals was determined to evaluate in vivo effect against H1N1. ELISA test was conducted to observe the effect on IFN-alpha, IFN-beta, IL-2, IL-6 and TNF-alpha in serum, as well as IFN-beta secretion in H1N1 infected MDCK supernatant. Real-time RT-PCR was employed to observe the expression levels of IRAK4 and TLR3 mRNA.

RESULT

The in vivo experiment shows that the hemagglutination titer was significantly decreased when the mice were treated with VOSH (0.266 mg x kg(-1)), menthone(0.5 mg x kg(-1)) and pulegone (0.19 mg x kg(-1)) in therapeutic way; VOSH (0.226 mg x kg(-1)) had a significant effect on increasing serum levels of IFN-alpha, IL-2; Methone (0.5 mg x kg(-1)) had a significant effect on increasing serum levels of IFN-beta; Methone (0.5 mg x kg(-1)) and pulegone (0.19 mg x kg(-1)) had a significant effect on decreasing serum levels of IL-6; VOSH (0.452, 0.226 mg x kg(-1)) and pulegone (0.19 mg x kg(-1)) had a significant effect on decreasing serum levels TNF-alpha. The in vitro experiment showed that the expression levels of IRAK4 mRNA and IFN-beta were significantly increased in VOHS (0.1 g x L(-1)) and pulegone (0.1 g x L(-1)) groups; and the menthone (0.25 g x L(-1)) group showed a significant rise in the expression levels of IRAK4 mRNA, but a notable decline in TLR3 mRNA.

CONCLUSION

The administration with VOSH, methone and pulegone in therapeutic way can significantly decrease the hemagglutination titer, which demonstrates the anti-virus effect of the administration in therapeutic way, but no notable efficacy of the administration in preventive way. The in vivo anti-virus mechanism is related to regulation of IFN-alpha, IFN-beta and IL-2.

摘要

目的

观察荆芥挥发油（VOSH）及其主要成分薄荷酮和胡薄荷酮对甲型流感病毒A/PR/8/34（H1N1）的体内外抗病毒作用，以及其Toll样受体/干扰素（TLR/IFN）信号传导机制。

方法

制备小鼠肺适应PR - 8病毒模型。给予预防性和治疗性药物，测定模型动物的血凝滴度以评估对H1N1的体内作用。进行ELISA试验以观察对血清中IFN -α、IFN -β、IL - 2、IL - 6和TNF -α的影响，以及H1N1感染的MDCK上清液中IFN -β的分泌。采用实时RT - PCR观察IRAK4和TLR3 mRNA的表达水平。

结果

体内实验表明，治疗组给予VOSH（0.266 mg·kg⁻¹）、薄荷酮（0.5 mg·kg⁻¹）和胡薄荷酮（0.19 mg·kg⁻¹）时，血凝滴度显著降低；VOSH（0.226 mg·kg⁻¹）对提高血清IFN -α、IL - 2水平有显著作用；薄荷酮（0.5 mg·kg⁻¹）对提高血清IFN -β水平有显著作用；薄荷酮（0.5 mg·kg⁻¹）和胡薄荷酮（0.19 mg·kg⁻¹）对降低血清IL - 6水平有显著作用；VOSH（0.452、0.226 mg·kg⁻¹）和胡薄荷酮（0.19 mg·kg⁻¹）对降低血清TNF -α水平有显著作用。体外实验表明，VOHS（0.1 g·L⁻¹）和胡薄荷酮（0.1 g·L⁻¹）组IRAK4 mRNA和IFN -β的表达水平显著升高；薄荷酮（0.25 g·L⁻¹）组IRAK4 mRNA表达水平显著升高，但TLR3 mRNA显著下降。