淫羊藿苷与染料木黄酮对骨髓基质细胞成骨活性的比较

[Comparison between icariin and genistein in osteogenic activity of marrow stromal cells].

作者信息

Zhou Jian, Chen Ke-Ming, Ge Bao-Feng, Ma Xiao-Ni, Guo Xiao-Yu, Cheng Kui, Gao Yu-Hai, Yan Li-Juan, Shi Wen-Gui

机构信息

Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2013 Jun;38(11):1783-8.

PMID:24010296

Abstract

OBJECTIVE

To compare the effect of icariin and genistein in the osteogenic differentiation of rat bone marrow stromal cells (rBMSC).

METHOD

Rat marrow stromal cells were seperated in vitro, and the optimal concentration of genisten and icriin were screened. Genistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) were adopted to intereven rBMSCs cultured in vitro. Alkaline phosphatase (ALP) was determined at 3, 6, 9, 12,15 d after intervention; calcified nodule was detected with alizarin red staining at 12 d; OXS, Runx-2, bone morphogenetic protein (BMP-2) and Collagen-I mRNA expression were observed with Real-time RT-PCR at 12, 24, 48, 72, 96 h.

RESULT

Genistein and icariin with the concentration of 1 x 10(-5) mol x L(-1) could increase the activity of ALP and the content of Ca, regulate OXS, BMP-2, Runx-2 and Collagen-I mRNA expression.

CONCLUSION

Icariin showed a stronger effect in improving the osteogenic differentiation of rat bone marrow stromal cells than genistein.

摘要

目的

比较淫羊藿苷和染料木黄酮对大鼠骨髓间充质干细胞（rBMSC）成骨分化的影响。

方法

体外分离大鼠骨髓间充质干细胞，筛选染料木黄酮和淫羊藿苷的最佳浓度。采用浓度为1×10⁻⁵ mol·L⁻¹的染料木黄酮和淫羊藿苷干预体外培养的rBMSC。干预后3、6、9、12、15 d测定碱性磷酸酶（ALP）；干预12 d时用茜素红染色检测钙化结节；在12、24、48、72、96 h用实时荧光定量逆转录聚合酶链反应（Real-time RT-PCR）观察氧化应激蛋白（OXS）、Runx-2、骨形态发生蛋白（BMP-2）和I型胶原（Collagen-I）mRNA表达。