解释性章节：PCR引物设计。

Explanatory chapter: PCR primer design.

作者信息

Álvarez-Fernández Rubén

机构信息

Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.

出版信息

Methods Enzymol. 2013;529:1-21. doi: 10.1016/B978-0-12-418687-3.00001-X.

DOI:10.1016/B978-0-12-418687-3.00001-X

PMID:24011032

Abstract

This chapter is intended as a guide on polymerase chain reaction (PCR) primer design (for information on PCR, see General PCR and Explanatory Chapter: Troubleshooting PCR). In the next section, general guidelines will be provided, followed by a discussion on primer design for specific applications. A list of recommended software tools is shown at the end.

摘要

本章旨在作为聚合酶链反应（PCR）引物设计的指南（有关PCR的信息，请参阅通用PCR和解释性章节：PCR故障排除）。在下一节中，将提供一般指南，随后讨论针对特定应用的引物设计。文末列出了推荐的软件工具清单。