Koh Cheryl M
Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods Enzymol. 2013;529:161-9. doi: 10.1016/B978-0-12-418687-3.00013-6.
The isolation of genomic DNA from mammalian cells is a routine molecular biology laboratory technique with numerous downstream applications. The isolated DNA can be used as a template for PCR, cloning, and genotyping and to generate genomic DNA libraries. It can also be used for sequencing to detect mutations and other alterations, and for DNA methylation analyses.
从哺乳动物细胞中分离基因组DNA是一项常规的分子生物学实验室技术，具有众多下游应用。分离出的DNA可作为PCR、克隆和基因分型的模板，并用于构建基因组DNA文库。它还可用于测序以检测突变和其他改变，以及进行DNA甲基化分析。
