Shenzhen Exit and Entry Inspection and Quarantine Bureau, Shenzhen, Guangdong 518001, China.
J Virol Methods. 2013 Dec;194(1-2):206-10. doi: 10.1016/j.jviromet.2013.08.028. Epub 2013 Sep 4.
Goldfish Haematopoietic Necrosis caused by Cyprinid herpesvirus 2 (CyHV-2) is a severe fish disease with high level of mortality. This is the first study on detection of this disease by loop-mediated isothermal amplification (LAMP). A set of six primers targeting terminase gene (accession no. EU349285.1) was determined after a serial of tests. Detection limit was 1.09 × 10(-4)μg/μL, which was superior to conventional PCR and real-time PCR. No cross reaction with 28 other viruses or bacteria commonly found in fish was observed. The application of commercial kit and instrument for the LAMP assay could reduce the risk of cross contamination, which is suitable for detection of infection under field conditions.
金鱼造血器官坏死症由鲤疱疹病毒 2 （CyHV-2）引起，是一种具有高死亡率的严重鱼类疾病。这是首次应用环介导等温扩增技术（LAMP）对该疾病进行检测的研究。通过一系列测试确定了针对终止酶基因（登录号 EU349285.1）的六组引物。检测限为 1.09×10（-4）μg/μL，优于常规 PCR 和实时 PCR。与鱼类中常见的 28 种其他病毒或细菌无交叉反应。LAMP 检测中使用商用试剂盒和仪器可以降低交叉污染的风险，适用于现场条件下的感染检测。
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