El-Matbouli Mansour, Rucker Ute, Soliman Hatem
Institute of Zoology, Fish Biology and Fish Diseases, Faculty of Veterinary Medicine, University of Munich, Germany.
Dis Aquat Organ. 2007 Oct 31;78(1):23-8. doi: 10.3354/dao01858.
Diagnostic tests with high analytical sensitivity are required to detect Cyprinid herpesvirus-3 (CyHV-3) in carriers that have been implicated in the dissemination of this important disease of koi and common carp Cyprinus carpio. A nested polymerase chain reaction (PCR) test was developed to detect CyHV-3 DNA in tissues of infected fish. The 2-round PCR amplified 529 and 379 bp segments of the CyHV-3 gene coding for the major capsid protein using specific external and internal primers, respectively. The PCR test did not amplify genomic DNA from either Cyprinid herpesvirus-1 (CyHV-1) or Cyprinid herpesvirus-2 (CyHV-2). The nested PCR test detected CyHV-3 DNA at a 10-fold lower concentration than a routinely used, 1-round test. Hence, the new method should provide more sensitive diagnosis of CyHV-3 infection among koi and carp populations.
需要具有高分析灵敏度的诊断测试来检测鲤疱疹病毒3型（CyHV-3），这种病毒存在于被认为是锦鲤和鲤鱼（Cyprinus carpio）这种重要疾病传播源的携带者体内。开发了一种巢式聚合酶链反应（PCR）测试来检测受感染鱼组织中的CyHV-3 DNA。两轮PCR分别使用特异性外部和内部引物扩增了编码主要衣壳蛋白的CyHV-3基因的529和379 bp片段。该PCR测试未扩增来自鲤疱疹病毒1型（CyHV-1）或鲤疱疹病毒2型（CyHV-2）的基因组DNA。巢式PCR测试检测CyHV-3 DNA的浓度比常规使用的一轮测试低10倍。因此，新方法应能为锦鲤和鲤鱼群体中的CyHV-3感染提供更灵敏的诊断。
