Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics (IBCCF), Federal University of Rio de Janeiro (UFRJ), Brazil.
Respir Physiol Neurobiol. 2013 Dec 1;189(3):484-90. doi: 10.1016/j.resp.2013.08.019. Epub 2013 Sep 4.
We compared the effects of oleanolic acid (OA) vs. dexamethasone on lung mechanics and histology, inflammation, and apoptosis in lung and distal organs in experimental sepsis. Seventy-eight BALB/c mice were randomly divided into two groups. Sepsis was induced by cecal ligation and puncture, while the control group underwent sham surgery. 1h after surgery, all animals were further randomized to receive saline (SAL), OA and dexamethasone (DEXA) intraperitoneally. Both OA and DEXA improved lung mechanics and histology, which were associated with fewer lung neutrophils and less cell apoptosis in lung, liver, and kidney than SAL. However, only animals in the DEXA group had lower levels of interleukin (IL)-6 and KC (murine analog of IL-8) in bronchoalveolar lavage fluid than SAL animals. Conversely, OA was associated with lower inducible nitric oxide synthase expression and higher superoxide dismutase than DEXA. In the experimental sepsis model employed herein, OA and DEXA reduced lung damage and distal organ apoptosis through distinct anti-inflammatory mechanisms.
我们比较了齐墩果酸（OA）与地塞米松对实验性脓毒症中肺力学和组织学、炎症和细胞凋亡的影响。78 只 BALB/c 小鼠随机分为两组。通过盲肠结扎和穿孔诱导脓毒症，而对照组接受假手术。手术后 1 小时，所有动物进一步随机接受腹腔注射生理盐水（SAL）、OA 和地塞米松（DEXA）。OA 和 DEXA 均改善了肺力学和组织学，与 SAL 相比，肺、肝和肾中的肺中性粒细胞和细胞凋亡减少。然而，只有 DEXA 组动物的支气管肺泡灌洗液中白细胞介素（IL）-6 和 KC（IL-8 的鼠类似物）水平低于 SAL 动物。相反，OA 与诱导型一氧化氮合酶的表达降低和超氧化物歧化酶的表达升高有关，而 DEXA 则相反。在本实验性脓毒症模型中，OA 和 DEXA 通过不同的抗炎机制减轻肺损伤和远端器官凋亡。
