Area of Chemical Engineering, Department of Engineering, University of Almería, 04120 Almería, Spain.
Area of Chemical Engineering, Department of Engineering, University of Almería, 04120 Almería, Spain.
Bioresour Technol. 2013 Nov;147:569-576. doi: 10.1016/j.biortech.2013.08.092. Epub 2013 Aug 23.
Mixotrophic cultures of Phaeodactylum tricornutum were carried out in bubble columns using fructose and glycerol in indoor fed-batch and semi-continuous modes. In the fed-batch cultures, different nutrient-addition strategies, combined with stepwise increments in the light intensity, were assayed. It was found that glycerol promoted significantly higher biomass productivity than fructose. A glycerol-induced photoinhibition that arrested the growth of P. tricornutun was also observed. As this was considered a limitation as regards transferring the fed-batch mode to outdoor conditions, this information was used to culture P. tricornutum in semi-continuous mode. Similar glycerol-induced photoinhibition was not observed in these cultures, even at highest dilution rates. Although the highest biomass (1.5 g L(-1) d(-1)) and EPA (40 mg L(-1) d(-1)) productivities found in the semi-continuous cultures were lower than those obtained photoautotrophically in outdoor photobioreactors, the findings showed that semi-continuous mode was an excellent candidate for transferring mixotrophic culture to an outdoor setting.
采用室内分批补料和半连续方式，在鼓泡塔中利用果糖和甘油对三角褐指藻进行兼养培养。在分批补料培养中，测定了不同的补料策略，结合逐步增加光照强度。结果发现，甘油比果糖显著提高了生物量生产力。还观察到甘油诱导的光抑制作用会阻止三角褐指藻的生长。由于这被认为是将分批补料模式转移到室外条件的限制，因此将此信息用于半连续模式培养三角褐指藻。即使在最高稀释率下，在这些培养物中也没有观察到类似的甘油诱导的光抑制。尽管在半连续培养中发现的最高生物量（1.5 g L(-1) d(-1)）和 EPA（40 mg L(-1) d(-1)）生产力低于在室外光生物反应器中光自养获得的值，但研究结果表明，半连续模式是将兼养培养转移到室外环境的绝佳候选者。
