Drapikowska Maria, Susek Karolina, Hasterok R, Szkudlarz P, Celka Z, Jackowiak B
Poznań University of Life Sciences Department of Ecology and Environmental Protection Poznań Poland.
Acta Biol Hung. 2013 Sep;64(3):352-63. doi: 10.1556/ABiol.64.2013.3.8.
Diploid Anthoxanthum odoratum and tetraploid A. aristatum were compared with respect to stomatal guard cell lengths, and stomatal density at adaxial and abaxial surfaces of the lamina. Further, the genome size of both species was determined by flow cytometry, and the number as well as the chromosomal distribution of 5S and 45S rDNAs were examined using FISH with ribosomal DNA (rDNA) probes. The average length of stomatal guard cells in A. odoratum was shown to be greater than that for A. aristatum, but the ranges overlapped. Moreover, reduction in stomatal frequency was found at higher ploidy levels.The genome size was 6.863 pg/2C DNA for A. aristatum and 13.252 pg/2C DNA for A. odoratum. A. aristatum has four sites of 5S rDNA in its root-tip meristematic cells, whereas A. odoratum has six. Both species have six sites of 45S rDNA. Chromosomal localization of the rDNA varied, which suggests that chromosome rearrangements took place during Anthoxanthum genome evolution.
对二倍体黄花茅和四倍体芒颖黄花茅的叶片近轴面和远轴面的气孔保卫细胞长度及气孔密度进行了比较。此外，通过流式细胞术测定了两个物种的基因组大小，并使用核糖体DNA（rDNA）探针通过荧光原位杂交（FISH）检测了5S和45S rDNA的数量及其染色体分布。结果表明，黄花茅气孔保卫细胞的平均长度大于芒颖黄花茅，但两者范围有重叠。此外，发现多倍体水平越高，气孔频率越低。芒颖黄花茅的基因组大小为6.863 pg/2C DNA，黄花茅为13.252 pg/2C DNA。芒颖黄花茅根尖分生组织细胞中有4个5S rDNA位点，而黄花茅有6个。两个物种都有6个45S rDNA位点。rDNA的染色体定位各不相同，这表明在黄花茅属基因组进化过程中发生了染色体重排。
Zotero 插件