Kimoto Hiroko, Touhara Kazushige
Biological Research Laboratories, R&D Division, Daiichi Sankyo Co., LTD., Tokyo, Japan.
Methods Mol Biol. 2013;1068:47-53. doi: 10.1007/978-1-62703-619-1_4.
Our search for a substance recognized by the vomeronasal neurons revealed that the extra-orbital lacrimal gland (ELG) isolated from adult male mice produced the male-specific peptide pheromone exocrine gland-secreting peptide 1 (ESP1). The following protocol reveals how ESP1 may be extracted from the ELG, purified using anion-exchange and reverse-phase high-performance liquid chromatography (HPLC), and analyzed by mass spectrometry. This protocol has been specifically designed for the purification of ESP1, but may be modified to isolate a variety of peptides from the exocrine glands. Peptides purified in this manner may help further define the molecular mechanisms underlying pheromone communication in the vomeronasal system.
我们对犁鼻神经元所识别物质的研究发现，从成年雄性小鼠分离出的眶外泪腺（ELG）可产生雄性特异性肽类信息素——外分泌腺分泌肽1（ESP1）。以下方案展示了如何从ELG中提取ESP1，通过阴离子交换和反相高效液相色谱（HPLC）进行纯化，并通过质谱分析。该方案是专门为纯化ESP1设计的，但可进行修改以从外分泌腺中分离多种肽类。以这种方式纯化的肽类可能有助于进一步明确犁鼻系统中信息素通讯的分子机制。
