Stewart Michael, Baker Cindy F, Sorensen Peter W
National Institute of Water & Atmospheric Research Ltd., Hamilton, New Zealand.
Methods Mol Biol. 2013;1068:55-69. doi: 10.1007/978-1-62703-619-1_5.
Pheromones are chemicals that pass between members of the same species that have inherent meaning. In the case of fish, pheromones are water-soluble and found in low concentrations. As such, sensitive and selective methods are needed to separate and analyze these pheromones from an environmental matrix that may contain many other chemicals. This chapter describes a generic method used to concentrate and identify these chemicals and two extremely sensitive and selective methods for analysis, namely, mass spectrometry and enzyme-linked immunosorbent assay.
信息素是同一物种成员之间传递的具有内在意义的化学物质。就鱼类而言，信息素是水溶性的，且浓度较低。因此，需要灵敏且具选择性的方法来从可能含有许多其他化学物质的环境基质中分离和分析这些信息素。本章描述了一种用于浓缩和鉴定这些化学物质的通用方法，以及两种极其灵敏且具选择性的分析方法，即质谱分析法和酶联免疫吸附测定法。
