基于质谱法的蛋白质乙酰化检测。
Mass spectrometry-based detection of protein acetylation.
作者信息
Li Yu, Silva Jeffrey C, Skinner Mary E, Lombard David B
机构信息
Cell Signaling Technology, Danvers, MA, USA.
出版信息
Methods Mol Biol. 2013;1077:81-104. doi: 10.1007/978-1-62703-637-5_6.
Abstract
Improved sample preparation techniques and increasingly sensitive mass spectrometry (MS) analysis have revolutionized the study of protein post-translational modifications (PTMs) (Rush et al., Nat Biotechnol 23:94-101, 2005). Here, we describe a general approach for immunopurification and MS-based identification of acetylated proteins in biological samples. This approach is useful to characterize changes in the acetylome in response to biological interventions (Schwer et al., Aging Cell 8:604-606, 2009).
摘要
改进的样品制备技术和日益灵敏的质谱（MS）分析彻底改变了蛋白质翻译后修饰（PTM）的研究（Rush等人，《自然生物技术》23:94 - 101，2005年）。在此，我们描述了一种用于免疫纯化和基于MS鉴定生物样品中乙酰化蛋白质的通用方法。这种方法对于表征乙酰化蛋白质组响应生物干预的变化很有用（Schwer等人，《衰老细胞》8:604 - 606，2009年）。
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