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本文引用的文献

1
Simple and efficient purification of Escherichia coli DNA polymerase V: cofactor requirements for optimal activity and processivity in vitro.大肠杆菌 DNA 聚合酶 V 的简单高效纯化：体外最佳活性和连续性的辅因子要求。
DNA Repair (Amst). 2012 Apr 1;11(4):431-40. doi: 10.1016/j.dnarep.2012.01.012. Epub 2012 Feb 15.
2
Rapid DNA-protein cross-linking and strand scission by an abasic site in a nucleosome core particle.碱基切除修复酶识别并结合到 abasic 位点。
Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22475-80. doi: 10.1073/pnas.1012860108. Epub 2010 Dec 13.
3
The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP.DNA聚合酶V的活性形式是UmuD'(2)C-RecA-ATP。
Nature. 2009 Jul 16;460(7253):359-63. doi: 10.1038/nature08178.
4
Hydrogen bonding contributes to the selectivity of nucleotide incorporation opposite an oxidized abasic lesion.氢键作用有助于核苷酸掺入氧化脱碱基损伤对面时的选择性。
J Am Chem Soc. 2008 May 14;130(19):6080-1. doi: 10.1021/ja801715c. Epub 2008 Apr 16.
5
Enhancing the "A-rule" of translesion DNA synthesis: promutagenic DNA synthesis using modified nucleoside triphosphates.增强跨损伤DNA合成的“A规则”：使用修饰的核苷三磷酸进行促诱变DNA合成。
Biochemistry. 2007 Dec 4;46(48):13752-61. doi: 10.1021/bi701328h. Epub 2007 Nov 6.
6
Replication of an oxidized abasic site in Escherichia coli by a dNTP-stabilized misalignment mechanism that reads upstream and downstream nucleotides.通过读取上游和下游核苷酸的dNTP稳定错配机制在大肠杆菌中复制氧化脱碱基位点。
Biochemistry. 2006 Apr 18;45(15):5048-56. doi: 10.1021/bi052276v.
7
Varying DNA base-pair size in subangstrom increments: evidence for a loose, not large, active site in low-fidelity Dpo4 polymerase.以亚埃增量变化的DNA碱基对大小：低保真Dpo4聚合酶中活性位点松散而非庞大的证据。
Biochemistry. 2006 Mar 7;45(9):2772-8. doi: 10.1021/bi051961z.
8
A comprehensive comparison of DNA replication past 2-deoxyribose and its tetrahydrofuran analog in Escherichia coli.大肠杆菌中DNA复制经过2-脱氧核糖及其四氢呋喃类似物的全面比较。
Nucleic Acids Res. 2004 Oct 11;32(18):5480-5. doi: 10.1093/nar/gkh873. Print 2004.
9
Mutagenic effects of 2-deoxyribonolactone in Escherichia coli. An abasic lesion that disobeys the A-rule.2-脱氧核糖内酯对大肠杆菌的诱变作用。一种违反A规则的无碱基损伤。
Biochemistry. 2004 Jun 1;43(21):6723-33. doi: 10.1021/bi049813g.
10
Crystallographic snapshots of a replicative DNA polymerase encountering an abasic site.复制性DNA聚合酶遇到无碱基位点时的晶体学快照。
EMBO J. 2004 Apr 7;23(7):1483-93. doi: 10.1038/sj.emboj.7600150. Epub 2004 Apr 1.

DNA 聚合酶 V 的动力学支持氧化脱碱基损伤在大肠杆菌中的指导性质。

DNA polymerase V kinetics support the instructive nature of an oxidized abasic lesion in Escherichia coli.

机构信息

Department of Chemistry, Johns Hopkins University , 3400 North Charles Street, Baltimore, Maryland 21218, United States.

出版信息

Biochemistry. 2013 Sep 17;52(37):6301-3. doi: 10.1021/bi400997h. Epub 2013 Sep 9.

DOI:10.1021/bi400997h
PMID:24015801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3817503/
Abstract

Translesion synthesis past an oxidized abasic site, 2-deoxyribonolactone, in Escherichia coli results in high levels of dG incorporation and is dependent upon DNA polymerase V (Pol V). Kinetic experiments performed here affirm that Pol V preferentially incorporates dG opposite 2-deoxyribonolactone (L). Pol V discriminates between dG and dA on the basis of the apparent KD, suggesting that L provides instructive structural information to the enzyme despite lacking a Watson-Crick base.

摘要

跨损伤合成越过氧化的碱基缺失部位 2-脱氧核糖酮内酯在大肠杆菌中导致高水平的 dG 掺入，并且依赖于 DNA 聚合酶 V（Pol V）。此处进行的动力学实验证实，Pol V 优先将 dG 掺入到 2-脱氧核糖酮内酯（L）的对面。Pol V 根据表观 KD 值区分 dG 和 dA，表明尽管 L 缺乏 Watson-Crick 碱基，但它为酶提供了有指导意义的结构信息。