[非O157产志贺毒素大肠杆菌分离株的脉冲场凝胶电泳分型]
[Pulsed-field gel electrophoresis typing on non-O157 Shiga toxin-producing Escherichia coli isolates].
作者信息
Jin Dong, Zhao Ai-lan, Bai Xiang-ning, Meng Qiong, Yu Bo, Yuan Xue-jiao, Xiong Yan-wen, Hou Xue-xin, Li Zhen-jun
机构信息
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2013 May;34(5):503-6.
OBJECTIVE
To establish a database and to understand the molecular epidemiological features of non-O157 Shiga toxin-producing Escherichia coli (STEC) isolates from different animal reservoirs and patients.
METHODS
Pulsed-field gel electrophoresis (PFGE) was performed according to the PulseNet protocol with minor modifications. A dendrogram was constructed using the BioNumerics.
RESULTS
Under the PulseNet protocol, 62 PFGE patterns were obtained from 76 non-O157 STEC isolates and then divided into A to M groups. Isolates from different sources were widely distributed in different groups, but were predominant seen in certain groups.
CONCLUSION
The non-O157 STEC isolates in China were highly polymorphic. PulseNet protocol seemed to be suitable for the typing of Chinese non-O157 STEC isolates.
目的
建立一个数据库，并了解来自不同动物宿主和患者的非O157产志贺毒素大肠杆菌（STEC）分离株的分子流行病学特征。
方法
脉冲场凝胶电泳（PFGE）按照PulseNet方案进行，并稍作修改。使用BioNumerics构建树形图。
结果
按照PulseNet方案，从76株非O157 STEC分离株中获得62种PFGE图谱，然后分为A至M组。来自不同来源的分离株广泛分布于不同组中，但在某些组中占主导地位。
结论
中国的非O157 STEC分离株具有高度多态性。PulseNet方案似乎适用于中国非O157 STEC分离株的分型。
Zotero 插件