Zhan Ying, Wang Yu-ping, Li Chao, Liu Shi-guo, Gao Qun
Department of Obstetrics, Qingdao University, Qingdao, China.
Zhonghua Fu Chan Ke Za Zhi. 2013 May;48(5):326-9.
To investigate the association between single nucleotide polymorphism (SNP) of macrophage migration inhibitory factor (MIF) gene-rs1007888 and the pathogenesis of gestational diabetes mellitus (GDM).
A total of 120 GDM pregnant women (GDM group) and 165 healthy pregnant women (control group) from Affiliated Hospital of Medical College, Qingdao University were recruited from June 2011 to July 2012. Their age, gestational week, height and weight were recorded. The levels of fasting blood glucose (FBG) and fasting insulin (FIN) were determined. Body mass index (BMI), the hemeostasis model assessment-insulin resistance (HOMA-IR) and hemeostasis model assessment-β cell function (HOMA-β) were calculated. DNA was extracted from fasting blood samples. SNP of MIF-rs1007888G/A was determined by DNA sequencing.The FBG, FIN, HOMA-IR and HOMA-β were compared between GDM group and the control group.They were also compared among pregnancies with different genotypes.
(1) GDM group had higher FBG, FIN and HOMA-IR levels, but lower HOMA-β than the control group (all P < 0.05). (2) MIF-rs1007888 SNP genotype frequencies of GG, GA and AA were 37.5%, 45.8% and 16.7%, and the allelic frequencies of G and A were 60.4%, 39.6% in GDM group; However, in the control group, the frequencies of GG, GA and AA were 26.1%, 54.5% and 19.4%, and the allelic frequencies of G and A were 53.3%, 46.7%, respectively.The distributions of MIF genotypes in GDM patients were significantly different from the healthy subjects (P < 0.05).No significant difference of MIF-rs1007888 allele distributions was observed between GDM group and the control group (P > 0.05). (3) The FBG, FIN and HOMA-IR in pregnant women with GG genotype were statistically higher than those with GA or AA genotypes, while HOMA-β was lower in women with GG genotype (all P < 0.05).
The SNP of MIF rs-1007888 was related to the insulin resistance and pancreatic β cell function of pregnant women. GG genotype of MIF-rs1007888 might be a genetic susceptible factor in the pathogenesis of GDM.
探讨巨噬细胞移动抑制因子（MIF）基因rs1007888单核苷酸多态性（SNP）与妊娠期糖尿病（GDM）发病机制之间的关联。
选取2011年6月至2012年7月在青岛大学医学院附属医院就诊的120例GDM孕妇（GDM组）和165例健康孕妇（对照组）。记录其年龄、孕周、身高和体重。测定空腹血糖（FBG）和空腹胰岛素（FIN）水平。计算体重指数（BMI）、稳态模型评估胰岛素抵抗（HOMA-IR）和稳态模型评估β细胞功能（HOMA-β）。从空腹血样中提取DNA。采用DNA测序法测定MIF-rs1007888G/A的SNP。比较GDM组和对照组之间的FBG、FIN、HOMA-IR和HOMA-β。还比较了不同基因型孕妇之间的上述指标。
（1）GDM组的FBG、FIN和HOMA-IR水平高于对照组，而HOMA-β低于对照组（均P<0.05）。（2）GDM组中MIF-rs1007888 SNP基因型GG、GA和AA的频率分别为37.5%、45.8%和16.7% , G和A等位基因频率分别为60.4%、39.6%；然而，对照组中GG、GA和AA的频率分别为26.1%、54.5%和19.4%，G和A等位基因频率分别为53.3%、46.7%。GDM患者中MIF基因型分布与健康受试者有显著差异（P < 0.05）。GDM组和对照组之间MIF-rs1007888等位基因分布无显著差异（P>0.05）。（3）GG基因型孕妇的FBG、FIN和HOMA-IR在统计学上高于GA或AA基因型孕妇，而GG基因型孕妇的HOMA-β较低（均P<0.05）。
MIF rs-1007888的SNP与孕妇的胰岛素抵抗和胰岛β细胞功能有关。MIF-rs1007888的GG基因型可能是GDM发病机制中的一个遗传易感因素。
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