Holder I A
Department of Surgery, University of Cincinnati, College of Medicine, Ohio.
J Burn Care Rehabil. 1990 Jul-Aug;11(4):301-4. doi: 10.1097/00004630-199007000-00005.
Various antimicrobial solutions for topical use on burn wounds tested, in vitro with a newly described wet disc assay, for their activity against bacterial isolates from patients with burns. The antimicrobial activity of triple antibiotic solution was shown to reside in the neomycin component. The activity of 2.5% mafenide hydrochloride solution was equal to that of 4% mafenide HCl and could be used clinically with significant savings and perhaps, fewer side effects. Chlorhexidine gluconate had broad antimicrobial activity in an 0.05% solution in water; however, dissolving the active ingredient in saline nullified its activity significantly. The wet disc assay appears to be a useful means by which to assess the efficacy of solutions for topical use.
用一种新描述的湿盘试验对各种用于烧伤创面局部使用的抗菌溶液进行体外测试，以检测其对烧伤患者分离出的细菌的活性。结果表明，三联抗生素溶液的抗菌活性存在于新霉素成分中。2.5%盐酸马法尼溶液的活性与4%盐酸马法尼溶液相当，可在临床上使用，能显著节省费用，且可能副作用更少。葡萄糖酸氯己定在0.05%的水溶液中具有广泛的抗菌活性；然而，将活性成分溶解在盐水中会显著使其活性失效。湿盘试验似乎是评估局部使用溶液疗效的一种有用方法。
