Tresyl-activated support for high-performance affinity chromatography.

A new activated support TSK gel Tresyl-5PW was evaluated for the coupling of antibodies, which was found to occur easily under mild conditions with high yields. Optimum coupling conditions were a 2-h reaction at 25 degrees C in 1 M phosphate buffer (pH 7.5) when 2-3 mg antibody/ml support is to be coupled and a 6-7-h reaction when ca. 10 mg antibody/ml support is to be coupled. When antibodies were coupled under these conditions, antibody coupling yields greater than 80% and antigen binding efficiencies of 70-80% were achieved, probably owing to a selective attachment of the Fc region of the antibodies. Antigens (human serum proteins) could be separated rapidly without denaturation on antibody-coupled Tresyl-5PW.