Jacobsson B, Carlström A, Platz A, Collins V P
Department of Pathology, Danderyd Hospital, Sweden.
J Clin Endocrinol Metab. 1990 Oct;71(4):875-80. doi: 10.1210/jcem-71-4-875.
Transthyretin (TTR) cDNA probes were used to determine the presence of TTR mRNA in Northern blots from rat, porcine, and human organs as well as from human endocrine tumors. We also used in vitro translation in our study of the human tissues. In accordance with previous findings, TTR mRNA was found in the choroid plexus and, to a lesser extent, in the liver of all three species. In addition low levels of TTR mRNA were identified in the rat and human pancreas. All of the endocrine pancreatic tumors (two glucagonomas, two insulinomas, and one nonfunctional tumor) and the gut carcinoid also contained TTR mRNA, whereas other endocrine tumors (two pheochromocytomas and one paraganglioma) and one adenocarcinoma of the pancreas were TTR mRNA negative. The level of TTR mRNA expression in most of the endocrine pancreatic tumors exceeded that in the liver. The in vitro translations produced pre-TTR molecules of similar size for all TTR mRNA-positive human organs and tumors.
转甲状腺素蛋白（TTR）cDNA探针用于在大鼠、猪和人类器官以及人类内分泌肿瘤的Northern印迹中确定TTR mRNA的存在。在对人体组织的研究中，我们还使用了体外翻译。与先前的研究结果一致，在所有三个物种的脉络丛中均发现了TTR mRNA，在肝脏中的表达较少。此外，在大鼠和人类胰腺中也鉴定出低水平的TTR mRNA。所有内分泌性胰腺肿瘤（2例胰高血糖素瘤、2例胰岛素瘤和1例无功能性肿瘤）以及肠道类癌也含有TTR mRNA，而其他内分泌肿瘤（2例嗜铬细胞瘤和1例副神经节瘤）以及1例胰腺腺癌TTR mRNA呈阴性。大多数内分泌性胰腺肿瘤中TTR mRNA的表达水平超过肝脏中的表达水平。对于所有TTR mRNA阳性的人体器官和肿瘤，体外翻译产生了大小相似的前TTR分子。
