Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran,
J Mol Neurosci. 2013 Oct;51(2):328-36. doi: 10.1007/s12031-013-0094-5. Epub 2013 Sep 11.
In this study, we examined phenotypic features of human Wharton's jelly mesenchymal stem cells-derived oligodendrocytes. We induced human WJMSCs to form OLs by signaling molecules including basic fibroblastic growth factor, platelet-derived growth factor-AA, and triiodothyronine hormone. Differentiated WJMSCs showed morphologic characteristics of an OL phenotype. Expression of surface markers and genes in oligodendrocyte precursor cells or OLs were analyzed by immunocytochemistry staining and real-time polymerase chain reaction, respectively. These results suggest that WJMSCs could be programmed to OLs and might provide a potential source for cell therapy in neurodegenerative disease.
在这项研究中，我们研究了人脐带来源的间充质干细胞源性少突胶质细胞的表型特征。我们通过碱性成纤维细胞生长因子、血小板衍生生长因子-AA 和三碘甲状腺原氨酸激素等信号分子诱导人 WJMSCs 形成 OLs。分化的 WJMSCs 表现出 OL 表型的形态特征。通过免疫细胞化学染色和实时聚合酶链反应分别分析少突胶质前体细胞或 OLs 中的表面标志物和基因的表达。这些结果表明，WJMSCs 可以被编程为 OLs，并且可能为神经退行性疾病的细胞治疗提供潜在的来源。
