Campbell B K, Scaramuzzi R J, Downing J A, Evans G
Department of Animal Husbandry, University of Sydney, NSW, Australia.
J Reprod Fertil. 1990 Jul;89(2):485-96. doi: 10.1530/jrf.0.0890485.
Mature Merino ewes in which the left ovary and its vascular pedicle had been autotransplanted to the neck were divided into control (N = 5) and immunized groups (N = 6). The immunized ewes were treated (2 ml s.c.) with Fecundin 1 and 4 weeks before the start of blood sampling. Ovarian and jugular venous blood was collected every 10 min at two stages of the follicular phase (21-27 h and 38-42 h after i.m. injection of 125 micrograms of a prostaglandin (PG) analogue) and during the mid-luteal phase (8 h at 15-min intervals). The ewes were monitored regularly for luteal function and preovulatory LH surges. Hormone concentrations and anti-androstenedione titres were assayed by RIA and ovarian secretion rates of oestradiol-17 beta, progesterone and androstenedione were determined. After the booster immunization, progesterone increased simultaneously with titre in immunized ewes, reaching 30 ng/ml at the time of PG injection when median titre was 1:10,000. All ewes responded to PG with LH surges 42-72 h later: 2 of the immunized ewes then had a second LH surge within 3-4 days at a time when peripheral progesterone values were 2-3 ng/ml. The frequency of steroid and LH pulses was greater in immunized ewes (P less than 0.05) during the luteal phase but not the follicular phase. The secretion rate of androstenedione was 6-10 times greater (19-37 ng/min; P less than 0.001) in immunized ewes at all sampling stages. Progesterone secretion rates were 3 times greater (16 micrograms/min; P less than 0.001) during the luteal phase in immunized ewes. The amplitude of oestradiol pulses was significantly reduced in immunized ewes (4.8 vs 2.1 ng/min at +24 h and 6.5 vs 2.8 ng/min at +40 h in control and immunized ewes, respectively: P less than 0.05) during the follicular phase. However, the mean secretion rate of oestradiol at each phase of the cycle was not significantly different between treatment groups. Analysis of bound and free steroid using polyethylene glycol showed that greater than 98% of peripheral and ovarian venous androstenedione and 86% of peripheral progesterone was bound in immunized ewes but there was no appreciable binding (less than 0.1%) in control ewes. Similarly, 50% of ovarian venous oestradiol was bound in immunized ewes compared to 15% in control ewes. We conclude that immunization against androstenedione increases the secretion rate of androstenedione and progesterone but not of oestradiol.(ABSTRACT TRUNCATED AT 400 WORDS)
将左侧卵巢及其血管蒂自体移植至颈部的成年美利奴母羊被分为对照组（N = 5）和免疫组（N = 6）。在开始采血前1周和4周，对免疫组母羊进行皮下注射（2 ml）Fecundin处理。在卵泡期的两个阶段（肌肉注射125微克前列腺素（PG）类似物后21 - 27小时和38 - 42小时）以及黄体中期（每隔15分钟采集8小时），每隔10分钟采集卵巢和颈静脉血。定期监测母羊的黄体功能和排卵前促黄体生成素（LH）高峰。通过放射免疫分析法测定激素浓度和抗雄烯二酮滴度，并测定雌二醇-17β、孕酮和雄烯二酮的卵巢分泌率。加强免疫后，免疫组母羊的孕酮与滴度同时升高，在注射PG时达到30 ng/ml，此时中位滴度为1:10,000。所有母羊在42 - 72小时后对PG产生LH高峰反应：2只免疫组母羊在3 - 4天内出现第二次LH高峰，此时外周孕酮值为2 - 3 ng/ml。在黄体期而非卵泡期，免疫组母羊的类固醇和LH脉冲频率更高（P < 0.05）。在所有采样阶段，免疫组母羊的雄烯二酮分泌率高6 - 10倍（19 - 37 ng/min；P < 0.001）。在黄体期，免疫组母羊的孕酮分泌率高3倍（16微克/分钟；P < 0.001）。在卵泡期，免疫组母羊的雌二醇脉冲幅度显著降低（对照组和免疫组在+24小时时分别为4.8 ng/min对2.1 ng/min，在+40小时时分别为6.5 ng/min对2.8 ng/min：P < 0.05）。然而，各治疗组在周期各阶段的雌二醇平均分泌率无显著差异。使用聚乙二醇对结合型和游离型类固醇进行分析表明，免疫组母羊外周和卵巢静脉中的雄烯二酮98%以上以及外周孕酮的86%被结合，但对照组母羊中无明显结合（< 0.1%）。同样，免疫组母羊卵巢静脉中的雌二醇50%被结合，而对照组母羊为15%。我们得出结论，针对雄烯二酮的免疫增加了雄烯二酮和孕酮的分泌率，但未增加雌二醇的分泌率。（摘要截短至400字）
