Zhang Su-Juan, Zhang Yong-Tai, Zhao Ji-Hui, Shen Li-Na, Shi Feng, Feng Nian-Ping
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Pharmazie. 2013 Aug;68(8):653-60.
In this study, we prepared solid lipid nanoparticles (TV-SLNs) loaded with toad venom extract and investigated their anti-tumor effects in vitro in HeLa and SKOV-3 cells. TV-SLNs were prepared using a cold homogenization technique, and the formulation was optimized by central composite design and response surface methods. The anti-tumor activities of TV-SLNs were evaluated by analyzing cell division and cell cycle distribution by using the MTT assay and flow cytometry. After incubation with TV-SLNs, the growth of both HeLa and SKOV-3 cells was inhibited significantly. The percentage of HeLa cells in G0/G1 phase decreased, whereas that in the S and G2/M phases increased. Thus, the S and G2/M phases were blocked after the incubation of HeLa cells with TV-SLNs for 24 h. In contrast, the percentage of SKOV-3 cells in G0/G1 phase increased and then decreased in S and G2/M phases, with the G0/G1 phase being blocked after incubation with TV-SLNs for 24 h. Our results demonstrate that TV-SLNs inhibited the fissiparism of HeLa and SKOV-3 cells in a time-and dose-dependent manner. TV-SLNs may be effective as a novel TV vaginal delivery system for the treatment of cervical and ovarian cancers.
在本研究中，我们制备了负载蟾蜍毒液提取物的固体脂质纳米粒（TV-SLNs），并在体外研究了它们对HeLa和SKOV-3细胞的抗肿瘤作用。采用冷均质技术制备TV-SLNs，并通过中心复合设计和响应面法对其配方进行优化。通过MTT法和流式细胞术分析细胞分裂和细胞周期分布，评估TV-SLNs的抗肿瘤活性。用TV-SLNs孵育后，HeLa和SKOV-3细胞的生长均受到显著抑制。HeLa细胞在G0/G1期的百分比下降，而在S期和G2/M期的百分比增加。因此，HeLa细胞与TV-SLNs孵育24小时后，S期和G2/M期被阻断。相比之下，SKOV-3细胞在G0/G1期的百分比增加，然后在S期和G2/M期下降，与TV-SLNs孵育24小时后G0/G1期被阻断。我们的结果表明，TV-SLNs以时间和剂量依赖性方式抑制HeLa和SKOV-3细胞的分裂。TV-SLNs作为一种新型的用于治疗宫颈癌和卵巢癌的蟾蜍毒液阴道给药系统可能是有效的。
