Denek Ziya, Erbil Guven, Ozbal Seda, Micili Serap Cilaker, Ozogul Candan
Department of Histology and Embryology, School of Medicine, Dokuz Eylul University, Inciralti, Izmir, Turkey.
Department of Histology and Embryology, School of Medicine, Dokuz Eylul University, Inciralti, Izmir, Turkey
Toxicol Ind Health. 2016 Jan;32(1):106-17. doi: 10.1177/0748233713498437. Epub 2013 Sep 10.
The herbicide itself and the degradation products are highly toxic on biological systems. The aim of this study is to investigate the potential toxic effects of trifluralin (TRF) on the urinary system of male rats and to investigate the protective effects of resveratrol (RSV) in TRF-induced urinary system damage. A total of 35 male Wistar rats were randomly divided into: (1) control group, (2) sham group, (3) low dose TRF group (0.8 g/kg/day), (4) high dose TRF group (2 g/kg/day) and (5) high dose TRF + RSV group 10 mg/kg/day. RSV was administered for 21 days by intragastric gavage at a dose of 10 mg/kg/day after induction of TRF. Kidney, ureter and urinary bladder tissue was examined using light microscopy and ultrastructurally. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling was performed to detect apoptosis. Superoxide dismutase (SOD), glutathion peroxidase (GPx) and malondialdehyde (MDA) levels were also evaluated biochemically for oxidative stress parameters. Histological evaluation showed that TRF increases apoptosis and oxidative stress, causes histological tissue damages and biochemical changes in the kidneys but does not cause any damage to the ureter and bladder. Treatment with RSV significantly attenuated tissue damage in the urinary system of rats. Apopitotic cells were significantly decreased in the treatment group. Additionally, treatment with RSV decreased SOD and GPx levels and increased MDA levels in the kidney tissue of animals subjected to TRF. These results show that RSV can significantly minimize histological damage and biochemical differences in treating TRF-induced kidney injury in rats.
除草剂本身及其降解产物对生物系统具有高毒性。本研究旨在探讨氟乐灵（TRF）对雄性大鼠泌尿系统的潜在毒性作用，并研究白藜芦醇（RSV）对TRF诱导的泌尿系统损伤的保护作用。总共35只雄性Wistar大鼠被随机分为：（1）对照组，（2）假手术组，（3）低剂量TRF组（0.8 g/kg/天），（4）高剂量TRF组（2 g/kg/天）和（5）高剂量TRF + RSV组（10 mg/kg/天）。在诱导TRF后，以10 mg/kg/天的剂量通过胃内灌胃给予RSV 21天。使用光学显微镜和超微结构检查肾脏、输尿管和膀胱组织。进行末端脱氧核苷酸转移酶介导的dUTP缺口末端标记以检测细胞凋亡。还对超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和丙二醛（MDA）水平进行生化评估以检测氧化应激参数。组织学评估表明，TRF增加细胞凋亡和氧化应激，导致肾脏组织学损伤和生化变化，但对输尿管和膀胱没有造成任何损伤。RSV治疗显著减轻了大鼠泌尿系统的组织损伤。治疗组凋亡细胞明显减少。此外，RSV治疗降低了接受TRF处理的动物肾脏组织中的SOD和GPx水平，并增加了MDA水平。这些结果表明，RSV在治疗TRF诱导的大鼠肾损伤时可显著减轻组织学损伤和生化差异。
