Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
College of Life Science, Sichuan Agricultural Universities, Ya'an, Sichuan 625014, China.
Genomics. 2013 Nov-Dec;102(5-6):472-8. doi: 10.1016/j.ygeno.2013.08.007. Epub 2013 Sep 7.
MicroRNAs (miRNAs) are non-coding RNAs that are approximately 20-22 nucleotides long. miRNAs have been shown to be important regulators that control a large variety of biological functions in eukaryotic cells. To investigate the roles of miRNAs in maize endosperm development, two small RNA libraries of maize endosperm at two developmental stages were sequenced. A total of 17,773,394 and 18,586,523 small RNA raw reads were obtained, respectively. Further analysis identified and characterized 95 known miRNAs belonging to 20 miRNA families. In addition, 18 novel miRNAs were identified and grouped into 11 families. Potential targets for 5 of the novel miRNA families were successfully predicted. We had also identified 12 corresponding miRNAs* of these novel miRNAs. In summary, we investigated expression patterns of miRNA in maize endosperm at key developmental stages and identified miRNAs that are likely to playing an important role in endosperm development.
微小 RNA（miRNA）是大约 20-22 个核苷酸长的非编码 RNA。已经证明，miRNA 是重要的调控因子，可控制真核细胞中大量的生物功能。为了研究 miRNA 在玉米胚乳发育中的作用，我们对两个不同发育阶段的玉米胚乳的两个小 RNA 文库进行了测序。分别获得了总共 17,773,394 和 18,586,523 个小 RNA 原始读数。进一步分析鉴定和表征了 95 个属于 20 个 miRNA 家族的已知 miRNA。此外，还鉴定了 18 个新的 miRNA，并将其分为 11 个家族。成功预测了 5 个新 miRNA 家族的潜在靶标。我们还鉴定了这些新 miRNA 的 12 个相应 miRNA*。总之，我们研究了关键发育阶段玉米胚乳中 miRNA 的表达模式，并鉴定了可能在胚乳发育中发挥重要作用的 miRNA。
