Department of Molecular Ecology; Max Planck Institute for Chemical Ecology; Jena, Germany.
Plant Signal Behav. 2013 Nov;8(11):e26365. doi: 10.4161/psb.26365. Epub 2013 Sep 10.
Previously, we identified a novel herbivore elicitor-regulated protein in Nicotiana attenuata (NaHER1) that is required to suppress abscisic acid (ABA) catabolism during herbivore attack and activate a full defense response against herbivores. ABA, in addition to its newly defined role in defense activation, mainly controls seed germination and stomatal function of land plants. Here we show that N. attenuata seeds silenced in the expression of NaHER1 by RNA interference (irHER1) accumulated less ABA during germination, and germinated faster on ABA-containing media compared to WT. Curiously, epidermal cells of irHER1 plants were wrinkled, possibly due to the previously demonstrated increase in transpiration of irHER1 plants that may affect turgor and cause wrinkling of the cells. We conclude that NaHER1 is a highly pleiotropic regulator of ABA responses in N. attenuata plants.
先前，我们鉴定了烟草(Nicotiana attenuata)中一种新型的取食诱导调节蛋白 NaHER1，它在取食过程中抑制脱落酸(ABA)的代谢，激活对食草动物的全面防御反应。ABA 除了其在防御激活中的新定义作用外，主要控制陆地植物的种子萌发和气孔功能。在这里，我们发现通过 RNA 干扰（irHER1）沉默 NaHER1 表达的烟草种子在萌发过程中积累的 ABA 较少，并且在含有 ABA 的培养基上比 WT 种子萌发更快。奇怪的是，irHER1 植株的表皮细胞起皱，可能是由于先前证明 irHER1 植株的蒸腾作用增加，这可能影响膨压并导致细胞起皱。我们得出结论，NaHER1 是烟草植物 ABA 反应的一个高度多效的调节剂。
